Reconstruction of Trabecular Tissue from Human Trabecular Cells as a Multicellular Spheroid

Toshihiko Matsuo, Nobuhiko Matsuo

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


To understand the development of the trabecular meshwork of the eye, floating cellular aggregates (multicellular spheroids) were formed from human trabecular cells in a non-adherent environment of culture and incubated for up to one month. Dissociated trabecular cells formed multicellular spheroids within one day in the non-adherent environment, and apoptosis continued to occur in the spheroids which had been initially filled with cells. The final structure after one month appeared as a meshwork of cells with large extracellular spaces. Epidermal and basic fibroblast growth factor (EGF and bFGF) protected trabecular cells in the spheroids from apoptosis and, as a result, kept the spheroids filled with cells even after one month. In the absence of excess EGF or bFGF, the multicellular spheroids grown in vitro from human trabecular cells mimicked the mesh-like structure of normal trabecular tissue. In constrast, under an excess of these growth factors, spheroids of high cellularity, resembling the abnormal trabecular tissues of patients with congenital glaucoma, were formed.

Original languageEnglish
Pages (from-to)213-218
Number of pages6
JournalActa medica Okayama
Issue number4
Publication statusPublished - Aug 1 1997


  • Basic fibroblast growth factor
  • Epidermal growth factor
  • Histology
  • Human trabecular cells
  • Multicellular spheroids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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