Reconstitution of the water-oxidizing complex in manganese-depleted photosystem II preparations using synthetic Mn complexes: A fluorine-19 NMR study of the reconstitution process

Toshi Nagata, Sergei K. Zharmukhamedov, Andrei A. Khorobrykh, Vyacheslav V. Klimov, Suleyman Allakhverdiev

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Reconstitution of Mn-depleted photosystem II (PSII) particles was examined with synthetic trinuclear Mn complexes of newly developed tripod ligands. Rates of the electron transfer and oxygen evolution were up to 74-86 and 52-56% of those measured in native PSII. These values are higher than those for the PSII reconstituted by MnCl2. The role of the tripod ligands during the reconstitution process was examined by 19F NMR. Due to the high NMR sensitivity of the 19F nucleus and the low abundance of fluorine atoms in natural PSII, it was possible to selectively observe the fluorine atoms on the tripod ligand. It was shown that the tripod ligands were released from the Mn complex after the reconstitution. We propose that the primary step in the reconstitution process is the prebinding of the Mn complex to the hydrophobic part of the PSII particle.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalPhotosynthesis Research
Volume98
Issue number1-3
DOIs
Publication statusPublished - Oct 1 2008
Externally publishedYes

Fingerprint

fluorine
Photosystem II Protein Complex
Fluorine
Manganese
photosystem II
manganese
Nuclear magnetic resonance
Water
Ligands
water
Atoms
electron transfer
Electrons
Oxygen
ligands

Keywords

  • Fluorine-19 NMR
  • Manganese complex
  • Photosystem II
  • Reconstitution
  • Water-oxidizing complex

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Reconstitution of the water-oxidizing complex in manganese-depleted photosystem II preparations using synthetic Mn complexes : A fluorine-19 NMR study of the reconstitution process. / Nagata, Toshi; Zharmukhamedov, Sergei K.; Khorobrykh, Andrei A.; Klimov, Vyacheslav V.; Allakhverdiev, Suleyman.

In: Photosynthesis Research, Vol. 98, No. 1-3, 01.10.2008, p. 277-284.

Research output: Contribution to journalArticle

Nagata, Toshi ; Zharmukhamedov, Sergei K. ; Khorobrykh, Andrei A. ; Klimov, Vyacheslav V. ; Allakhverdiev, Suleyman. / Reconstitution of the water-oxidizing complex in manganese-depleted photosystem II preparations using synthetic Mn complexes : A fluorine-19 NMR study of the reconstitution process. In: Photosynthesis Research. 2008 ; Vol. 98, No. 1-3. pp. 277-284.
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