Abstract
Reconstitution of Mn-depleted photosystem II (PSII) particles was examined with synthetic trinuclear Mn complexes of newly developed tripod ligands. Rates of the electron transfer and oxygen evolution were up to 74-86 and 52-56% of those measured in native PSII. These values are higher than those for the PSII reconstituted by MnCl2. The role of the tripod ligands during the reconstitution process was examined by 19F NMR. Due to the high NMR sensitivity of the 19F nucleus and the low abundance of fluorine atoms in natural PSII, it was possible to selectively observe the fluorine atoms on the tripod ligand. It was shown that the tripod ligands were released from the Mn complex after the reconstitution. We propose that the primary step in the reconstitution process is the prebinding of the Mn complex to the hydrophobic part of the PSII particle.
| Original language | English |
|---|---|
| Pages (from-to) | 277-284 |
| Number of pages | 8 |
| Journal | Photosynthesis Research |
| Volume | 98 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Oct 1 2008 |
| Externally published | Yes |
Fingerprint
Keywords
- Fluorine-19 NMR
- Manganese complex
- Photosystem II
- Reconstitution
- Water-oxidizing complex
ASJC Scopus subject areas
- Biochemistry
- Plant Science
- Cell Biology
Cite this
Reconstitution of the water-oxidizing complex in manganese-depleted photosystem II preparations using synthetic Mn complexes : A fluorine-19 NMR study of the reconstitution process. / Nagata, Toshi; Zharmukhamedov, Sergei K.; Khorobrykh, Andrei A.; Klimov, Vyacheslav V.; Allakhverdiev, Suleyman.
In: Photosynthesis Research, Vol. 98, No. 1-3, 01.10.2008, p. 277-284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reconstitution of the water-oxidizing complex in manganese-depleted photosystem II preparations using synthetic Mn complexes
T2 - A fluorine-19 NMR study of the reconstitution process
AU - Nagata, Toshi
AU - Zharmukhamedov, Sergei K.
AU - Khorobrykh, Andrei A.
AU - Klimov, Vyacheslav V.
AU - Allakhverdiev, Suleyman
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Reconstitution of Mn-depleted photosystem II (PSII) particles was examined with synthetic trinuclear Mn complexes of newly developed tripod ligands. Rates of the electron transfer and oxygen evolution were up to 74-86 and 52-56% of those measured in native PSII. These values are higher than those for the PSII reconstituted by MnCl2. The role of the tripod ligands during the reconstitution process was examined by 19F NMR. Due to the high NMR sensitivity of the 19F nucleus and the low abundance of fluorine atoms in natural PSII, it was possible to selectively observe the fluorine atoms on the tripod ligand. It was shown that the tripod ligands were released from the Mn complex after the reconstitution. We propose that the primary step in the reconstitution process is the prebinding of the Mn complex to the hydrophobic part of the PSII particle.
AB - Reconstitution of Mn-depleted photosystem II (PSII) particles was examined with synthetic trinuclear Mn complexes of newly developed tripod ligands. Rates of the electron transfer and oxygen evolution were up to 74-86 and 52-56% of those measured in native PSII. These values are higher than those for the PSII reconstituted by MnCl2. The role of the tripod ligands during the reconstitution process was examined by 19F NMR. Due to the high NMR sensitivity of the 19F nucleus and the low abundance of fluorine atoms in natural PSII, it was possible to selectively observe the fluorine atoms on the tripod ligand. It was shown that the tripod ligands were released from the Mn complex after the reconstitution. We propose that the primary step in the reconstitution process is the prebinding of the Mn complex to the hydrophobic part of the PSII particle.
KW - Fluorine-19 NMR
KW - Manganese complex
KW - Photosystem II
KW - Reconstitution
KW - Water-oxidizing complex
UR - http://www.scopus.com/inward/record.url?scp=57849106329&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=57849106329&partnerID=8YFLogxK
U2 - 10.1007/s11120-008-9319-9
DO - 10.1007/s11120-008-9319-9
M3 - Article
C2 - 18581251
AN - SCOPUS:57849106329
VL - 98
SP - 277
EP - 284
JO - Photosynthesis Research
JF - Photosynthesis Research
SN - 0166-8595
IS - 1-3
ER -