Recombinant guinea pig CCL5 (RANTES) differentially modulates cytokine production in alveolar and peritoneal macrophages

Troy A. Skwor, Hyosun Cho, Craig Cassidy, Teizo Yoshimura, David N. McMurray

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The CC chemokine ligand 5 (CCL5; regulated on activation, normal T expressed and secreted) is known to recruit and activate leukocytes; however, its role in altering the responses of host cells to a subsequent encounter with a microbial pathogen has rarely been studied. Recombinant guinea pig (rgp)CCL5 was prepared, and its influence on peritoneal and alveolar macrophage activation was examined by measuring cytokine and chemokine mRNA expression in cells stimulated with rgpCCL5 alone or exposed to rgpCCL5 prior to lipopolysaccharide (LPS) stimulation. Levels of mRNA for guinea pig tumor necrosis factor α (TNF-α), interleukin (IL)-1β, CCL2 (monocyte chemoattractant protein-1), and CXC chemokine ligand 8 (IL-8) were analyzed by reverse transcription followed by real-time polymerase chain reaction analysis using SYBR Green. Bioactive TNF-α protein concentration was measured using the L929 bioassay. Both macrophage populations displayed significant enhancement of all the genes and TNF-α protein levels when stimulated with rgpCCL5, except for CCL2 in alveolar macrophages. When peritoneal or alveolar macrophages were pretreated with rgpCCL5 for 2 h and then exposed to low concentrations of LPS, diminished cytokine and chemokine mRNA levels were apparent at 6 h compared with LPS alone. At the protein level, there was a reduction in TNF-α protein at 6 h in the CCL5-pretreated cells compared with LPS alone. These results further support a role for CCL5 in macrophage activation in addition to chemotactic properties and suggest a role in regulating the inflammatory response to LPS in the guinea pig by modulating the production of proinflammatory cytokines by macrophages.

Original languageEnglish
Pages (from-to)1229-1239
Number of pages11
JournalJournal of Leukocyte Biology
Volume76
Issue number6
DOIs
Publication statusPublished - Dec 2004
Externally publishedYes

Fingerprint

Chemokine CCL5
Alveolar Macrophages
Peritoneal Macrophages
Lipopolysaccharides
Guinea Pigs
Cytokines
Tumor Necrosis Factor-alpha
Macrophage Activation
Chemokines
Messenger RNA
Proteins
Macrophages
Ligands
CXC Chemokines
CC Chemokines
Chemokine CCL2
Interleukin-8
Interleukin-1
Biological Assay
Reverse Transcription

Keywords

  • CCL2
  • CXCL8
  • IL-1β
  • Lipopolysacchadde
  • TNF-α

ASJC Scopus subject areas

  • Cell Biology

Cite this

Recombinant guinea pig CCL5 (RANTES) differentially modulates cytokine production in alveolar and peritoneal macrophages. / Skwor, Troy A.; Cho, Hyosun; Cassidy, Craig; Yoshimura, Teizo; McMurray, David N.

In: Journal of Leukocyte Biology, Vol. 76, No. 6, 12.2004, p. 1229-1239.

Research output: Contribution to journalArticle

Skwor, Troy A. ; Cho, Hyosun ; Cassidy, Craig ; Yoshimura, Teizo ; McMurray, David N. / Recombinant guinea pig CCL5 (RANTES) differentially modulates cytokine production in alveolar and peritoneal macrophages. In: Journal of Leukocyte Biology. 2004 ; Vol. 76, No. 6. pp. 1229-1239.
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