Recombinant core protein fragment of phosphacan, a brain specific chondroitin sulfate proteoglycan, promote excitotoxic cell death of cultured rat hippocampal neurons

Sekiko Kurazono, Motoi Okamoto, Shuji Mori, Hideki Matsui

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated the role of phosphacan, a chondroitin sulfate proteoglycan that is constitutively expressed in the adult hippocampus, and recombinant core proteins of phosphacan in excitotoxic cell death of primary cultured rat hippocampal neurons. Phosphacan had no significant effect on excitotoxic neuronal death. Surprisingly, one of three recombinant proteins corresponding to N-terminal portions of phosphacan core protein dramatically promoted excitotoxic neuronal death. Moreover, the recombinant protein induced cell death of rat hippocampal neurons, even when neurons were not exposed to glutamate. These results suggest that proteolytic degradation of phosphacan and resultant core protein fragments may contribute to neuronal degeneration of hippocampal neurons in various neuropathological conditions.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalNeuroscience Letters
Volume304
Issue number3
DOIs
Publication statusPublished - May 25 2001

Fingerprint

Class 5 Receptor-Like Protein Tyrosine Phosphatases
Chondroitin Sulfate Proteoglycans
Recombinant Proteins
Cell Death
Neurons
Brain
Nerve Degeneration
Glutamic Acid
Hippocampus
Proteins
urinary gonadotropin fragment

Keywords

  • Cell culture
  • Chondroitin sulfate
  • Core protein
  • Hippocampus
  • Neurotoxicity
  • Phosphacan
  • Proteoglycan

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Recombinant core protein fragment of phosphacan, a brain specific chondroitin sulfate proteoglycan, promote excitotoxic cell death of cultured rat hippocampal neurons. / Kurazono, Sekiko; Okamoto, Motoi; Mori, Shuji; Matsui, Hideki.

In: Neuroscience Letters, Vol. 304, No. 3, 25.05.2001, p. 169-172.

Research output: Contribution to journalArticle

@article{8a39b72d728f48b8bb966660e138de13,
title = "Recombinant core protein fragment of phosphacan, a brain specific chondroitin sulfate proteoglycan, promote excitotoxic cell death of cultured rat hippocampal neurons",
abstract = "We investigated the role of phosphacan, a chondroitin sulfate proteoglycan that is constitutively expressed in the adult hippocampus, and recombinant core proteins of phosphacan in excitotoxic cell death of primary cultured rat hippocampal neurons. Phosphacan had no significant effect on excitotoxic neuronal death. Surprisingly, one of three recombinant proteins corresponding to N-terminal portions of phosphacan core protein dramatically promoted excitotoxic neuronal death. Moreover, the recombinant protein induced cell death of rat hippocampal neurons, even when neurons were not exposed to glutamate. These results suggest that proteolytic degradation of phosphacan and resultant core protein fragments may contribute to neuronal degeneration of hippocampal neurons in various neuropathological conditions.",
keywords = "Cell culture, Chondroitin sulfate, Core protein, Hippocampus, Neurotoxicity, Phosphacan, Proteoglycan",
author = "Sekiko Kurazono and Motoi Okamoto and Shuji Mori and Hideki Matsui",
year = "2001",
month = "5",
day = "25",
doi = "10.1016/S0304-3940(01)01778-5",
language = "English",
volume = "304",
pages = "169--172",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Recombinant core protein fragment of phosphacan, a brain specific chondroitin sulfate proteoglycan, promote excitotoxic cell death of cultured rat hippocampal neurons

AU - Kurazono, Sekiko

AU - Okamoto, Motoi

AU - Mori, Shuji

AU - Matsui, Hideki

PY - 2001/5/25

Y1 - 2001/5/25

N2 - We investigated the role of phosphacan, a chondroitin sulfate proteoglycan that is constitutively expressed in the adult hippocampus, and recombinant core proteins of phosphacan in excitotoxic cell death of primary cultured rat hippocampal neurons. Phosphacan had no significant effect on excitotoxic neuronal death. Surprisingly, one of three recombinant proteins corresponding to N-terminal portions of phosphacan core protein dramatically promoted excitotoxic neuronal death. Moreover, the recombinant protein induced cell death of rat hippocampal neurons, even when neurons were not exposed to glutamate. These results suggest that proteolytic degradation of phosphacan and resultant core protein fragments may contribute to neuronal degeneration of hippocampal neurons in various neuropathological conditions.

AB - We investigated the role of phosphacan, a chondroitin sulfate proteoglycan that is constitutively expressed in the adult hippocampus, and recombinant core proteins of phosphacan in excitotoxic cell death of primary cultured rat hippocampal neurons. Phosphacan had no significant effect on excitotoxic neuronal death. Surprisingly, one of three recombinant proteins corresponding to N-terminal portions of phosphacan core protein dramatically promoted excitotoxic neuronal death. Moreover, the recombinant protein induced cell death of rat hippocampal neurons, even when neurons were not exposed to glutamate. These results suggest that proteolytic degradation of phosphacan and resultant core protein fragments may contribute to neuronal degeneration of hippocampal neurons in various neuropathological conditions.

KW - Cell culture

KW - Chondroitin sulfate

KW - Core protein

KW - Hippocampus

KW - Neurotoxicity

KW - Phosphacan

KW - Proteoglycan

UR - http://www.scopus.com/inward/record.url?scp=0035947430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035947430&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(01)01778-5

DO - 10.1016/S0304-3940(01)01778-5

M3 - Article

C2 - 11343829

AN - SCOPUS:0035947430

VL - 304

SP - 169

EP - 172

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -