Recombinant core protein fragment of phosphacan, a brain specific chondroitin sulfate proteoglycan, promote excitotoxic cell death of cultured rat hippocampal neurons

Sekiko Kurazono, Motoi Okamoto, Shuji Mori, Hideki Matsui

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated the role of phosphacan, a chondroitin sulfate proteoglycan that is constitutively expressed in the adult hippocampus, and recombinant core proteins of phosphacan in excitotoxic cell death of primary cultured rat hippocampal neurons. Phosphacan had no significant effect on excitotoxic neuronal death. Surprisingly, one of three recombinant proteins corresponding to N-terminal portions of phosphacan core protein dramatically promoted excitotoxic neuronal death. Moreover, the recombinant protein induced cell death of rat hippocampal neurons, even when neurons were not exposed to glutamate. These results suggest that proteolytic degradation of phosphacan and resultant core protein fragments may contribute to neuronal degeneration of hippocampal neurons in various neuropathological conditions.

Original languageEnglish
Pages (from-to)169-172
Number of pages4
JournalNeuroscience Letters
Volume304
Issue number3
DOIs
Publication statusPublished - May 25 2001

Keywords

  • Cell culture
  • Chondroitin sulfate
  • Core protein
  • Hippocampus
  • Neurotoxicity
  • Phosphacan
  • Proteoglycan

ASJC Scopus subject areas

  • Neuroscience(all)

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