Recombinant aryl hydrocarbon receptors for bioassay of aryl hydrocarbon receptor ligands in transgenic tobacco plants

Susumu Kodama, Kumiko Okada, Keiko Akimoto, Hideyuki Inui, Hideo Ohkawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, β-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the β-glucuronidase reporter gene. The results clearly suggest that XDVs are functional transcription factors that respond to AhR ligands, and that the XDV-mediated reporter gene expression system is applicable to bioassays for dioxin residues in the environment.

Original languageEnglish
Pages (from-to)119-128
Number of pages10
JournalPlant Biotechnology Journal
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 2009
Externally publishedYes

Fingerprint

Aryl Hydrocarbon Receptors
Dioxins
dioxins
Genetically Modified Plants
Reporter Genes
reporter genes
Biological Assay
Tobacco
hydrocarbons
tobacco
bioassays
genetically modified organisms
Ligands
receptors
Herpes Simplex Virus Protein Vmw65
Indigo Carmine
indigo
Repressor Proteins
Gene Expression
herpes simplex

Keywords

  • AhR ligands
  • Aryl hydrocarbon receptors
  • Bioassay
  • Inducible gene expression
  • Transgenic plants

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology
  • Agronomy and Crop Science

Cite this

Recombinant aryl hydrocarbon receptors for bioassay of aryl hydrocarbon receptor ligands in transgenic tobacco plants. / Kodama, Susumu; Okada, Kumiko; Akimoto, Keiko; Inui, Hideyuki; Ohkawa, Hideo.

In: Plant Biotechnology Journal, Vol. 7, No. 2, 02.2009, p. 119-128.

Research output: Contribution to journalArticle

Kodama, Susumu ; Okada, Kumiko ; Akimoto, Keiko ; Inui, Hideyuki ; Ohkawa, Hideo. / Recombinant aryl hydrocarbon receptors for bioassay of aryl hydrocarbon receptor ligands in transgenic tobacco plants. In: Plant Biotechnology Journal. 2009 ; Vol. 7, No. 2. pp. 119-128.
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