Abstract
Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, β-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the β-glucuronidase reporter gene. The results clearly suggest that XDVs are functional transcription factors that respond to AhR ligands, and that the XDV-mediated reporter gene expression system is applicable to bioassays for dioxin residues in the environment.
| Original language | English |
|---|---|
| Pages (from-to) | 119-128 |
| Number of pages | 10 |
| Journal | Plant Biotechnology Journal |
| Volume | 7 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2009 |
| Externally published | Yes |
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Keywords
- AhR ligands
- Aryl hydrocarbon receptors
- Bioassay
- Inducible gene expression
- Transgenic plants
ASJC Scopus subject areas
- Plant Science
- Biotechnology
- Agronomy and Crop Science
Cite this
Recombinant aryl hydrocarbon receptors for bioassay of aryl hydrocarbon receptor ligands in transgenic tobacco plants. / Kodama, Susumu; Okada, Kumiko; Akimoto, Keiko; Inui, Hideyuki; Ohkawa, Hideo.
In: Plant Biotechnology Journal, Vol. 7, No. 2, 02.2009, p. 119-128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Recombinant aryl hydrocarbon receptors for bioassay of aryl hydrocarbon receptor ligands in transgenic tobacco plants
AU - Kodama, Susumu
AU - Okada, Kumiko
AU - Akimoto, Keiko
AU - Inui, Hideyuki
AU - Ohkawa, Hideo
PY - 2009/2
Y1 - 2009/2
N2 - Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, β-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the β-glucuronidase reporter gene. The results clearly suggest that XDVs are functional transcription factors that respond to AhR ligands, and that the XDV-mediated reporter gene expression system is applicable to bioassays for dioxin residues in the environment.
AB - Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, β-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the β-glucuronidase reporter gene. The results clearly suggest that XDVs are functional transcription factors that respond to AhR ligands, and that the XDV-mediated reporter gene expression system is applicable to bioassays for dioxin residues in the environment.
KW - AhR ligands
KW - Aryl hydrocarbon receptors
KW - Bioassay
KW - Inducible gene expression
KW - Transgenic plants
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UR - http://www.scopus.com/inward/citedby.url?scp=58149392479&partnerID=8YFLogxK
U2 - 10.1111/j.1467-7652.2008.00378.x
DO - 10.1111/j.1467-7652.2008.00378.x
M3 - Article
C2 - 19055610
AN - SCOPUS:58149392479
VL - 7
SP - 119
EP - 128
JO - Plant Biotechnology Journal
JF - Plant Biotechnology Journal
SN - 1467-7644
IS - 2
ER -