Recombinant α-actin for specific fluorescent labeling

Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Until recently, actin was thought to act merely as a passive track for its motility partner, myosin, during actomyosin interactions. Yet a recent report having observed dynamical conformational changes in labeled skeletal muscle a-actin suggests that actin has a more active role. Because the labeling technique was still immature, however, conclusions regarding the signi-ficance of the different conformations are difficult to make. Here, we describe the preparation of fully active a-actin obtained from a baculovirus expression system. We developed a-actin recombinants, of which subdomains 1 and 2 have specific sites for fluorescent probes. This specific labeling technique offers to significantly expand the information acquired from actin studies.

Original languageEnglish
Pages (from-to)491-499
Number of pages9
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume85
Issue number10
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

Fingerprint

fluorescent labeling
marking
actin
Actins
myosins
skeletal muscle
locomotion
labeling techniques
preparation
probes
Actomyosin
Baculoviridae
interactions
Myosins
myosin
Fluorescent Dyes
Skeletal Muscle
immatures

Keywords

  • α-Actin
  • Actomyosin
  • Fluorescent imaging
  • In vitro motility assay
  • Polymerization
  • Recombinant protein

ASJC Scopus subject areas

  • General

Cite this

Recombinant α-actin for specific fluorescent labeling. / Iwane, Atsuko H.; Morimatsu, Masatoshi; Yanagida, Toshio.

In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 85, No. 10, 12.2009, p. 491-499.

Research output: Contribution to journalArticle

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