Recombinant α-actin for specific fluorescent labeling

Atsuko H. Iwane, Masatoshi Morimatsu, Toshio Yanagida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Until recently, actin was thought to act merely as a passive track for its motility partner, myosin, during actomyosin interactions. Yet a recent report having observed dynamical conformational changes in labeled skeletal muscle a-actin suggests that actin has a more active role. Because the labeling technique was still immature, however, conclusions regarding the signi-ficance of the different conformations are difficult to make. Here, we describe the preparation of fully active a-actin obtained from a baculovirus expression system. We developed a-actin recombinants, of which subdomains 1 and 2 have specific sites for fluorescent probes. This specific labeling technique offers to significantly expand the information acquired from actin studies.

Original languageEnglish
Pages (from-to)491-499
Number of pages9
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume85
Issue number10
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Keywords

  • Actomyosin
  • Fluorescent imaging
  • In vitro motility assay
  • Polymerization
  • Recombinant protein
  • α-Actin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

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