Reciprocal gene expression of rat fibroglycan and β-actin during the course of regeneration after D-galactosamine liver injury

Toshiyuki Shinji, Norio Koide, Hajime Hada, Shunsuke Sasaki, Takahiko Oka, Niro Takayama, Hidenori Shiraha, Kozo Ujike, Masaki Nakamura, Tadashi Hanafusa, Yasuhiro Yumoto, Takao Tsuji

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fibroglycan (FG) is a major heparan sulfate proteoglycan (HSPG) in the rat liver that is mainly distributed on the surface of the hepatocytes. HSPG may play some important roles in the regeneration of liver by interacting with various growth factors such as bFGF and HB-EGF. However, little is known about the function of FG. We reported that after injury caused by D-galactosamine, regeneration started on the following day and peaked on day 2. To clarify the function of FG in liver regeneration, we investigated the gene expression of FG during regeneration after D-galactosamine injury. Materials and Methods: Rats were given D-galactosamine on day 0. Liver RNA was collected from day 0 to day 7. The gene expression of FG and β-actin (as a representative cytoskeleton) was examined by Northern and/or Slot blotting. Results: FG gene expression was markedly decreased on day 2, but totally recovered on day 3. In contrast, β-actin gene expression was markedly increased on day 2 and returned to the normal level on day 3. Expression of the FG and β-actin genes was reciprocal. Conclusion: FG expression is transiently suppressed when cytoskeleton gene expression is enhanced at the early phase of liver regeneration.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalHepato-Gastroenterology
Volume44
Issue number13
Publication statusPublished - 1997

Fingerprint

Syndecan-2
Galactosamine
Actins
Regeneration
Gene Expression
Liver
Wounds and Injuries
Liver Regeneration
Heparan Sulfate Proteoglycans
Cytoskeleton
Hepatocytes
Intercellular Signaling Peptides and Proteins
RNA

Keywords

  • B-actin
  • D-galactosamine injury
  • Fibroglycan
  • mRNA expression
  • Rat liver

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Shinji, T., Koide, N., Hada, H., Sasaki, S., Oka, T., Takayama, N., ... Tsuji, T. (1997). Reciprocal gene expression of rat fibroglycan and β-actin during the course of regeneration after D-galactosamine liver injury. Hepato-Gastroenterology, 44(13), 239-244.

Reciprocal gene expression of rat fibroglycan and β-actin during the course of regeneration after D-galactosamine liver injury. / Shinji, Toshiyuki; Koide, Norio; Hada, Hajime; Sasaki, Shunsuke; Oka, Takahiko; Takayama, Niro; Shiraha, Hidenori; Ujike, Kozo; Nakamura, Masaki; Hanafusa, Tadashi; Yumoto, Yasuhiro; Tsuji, Takao.

In: Hepato-Gastroenterology, Vol. 44, No. 13, 1997, p. 239-244.

Research output: Contribution to journalArticle

Shinji, T, Koide, N, Hada, H, Sasaki, S, Oka, T, Takayama, N, Shiraha, H, Ujike, K, Nakamura, M, Hanafusa, T, Yumoto, Y & Tsuji, T 1997, 'Reciprocal gene expression of rat fibroglycan and β-actin during the course of regeneration after D-galactosamine liver injury', Hepato-Gastroenterology, vol. 44, no. 13, pp. 239-244.
Shinji, Toshiyuki ; Koide, Norio ; Hada, Hajime ; Sasaki, Shunsuke ; Oka, Takahiko ; Takayama, Niro ; Shiraha, Hidenori ; Ujike, Kozo ; Nakamura, Masaki ; Hanafusa, Tadashi ; Yumoto, Yasuhiro ; Tsuji, Takao. / Reciprocal gene expression of rat fibroglycan and β-actin during the course of regeneration after D-galactosamine liver injury. In: Hepato-Gastroenterology. 1997 ; Vol. 44, No. 13. pp. 239-244.
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abstract = "Fibroglycan (FG) is a major heparan sulfate proteoglycan (HSPG) in the rat liver that is mainly distributed on the surface of the hepatocytes. HSPG may play some important roles in the regeneration of liver by interacting with various growth factors such as bFGF and HB-EGF. However, little is known about the function of FG. We reported that after injury caused by D-galactosamine, regeneration started on the following day and peaked on day 2. To clarify the function of FG in liver regeneration, we investigated the gene expression of FG during regeneration after D-galactosamine injury. Materials and Methods: Rats were given D-galactosamine on day 0. Liver RNA was collected from day 0 to day 7. The gene expression of FG and β-actin (as a representative cytoskeleton) was examined by Northern and/or Slot blotting. Results: FG gene expression was markedly decreased on day 2, but totally recovered on day 3. In contrast, β-actin gene expression was markedly increased on day 2 and returned to the normal level on day 3. Expression of the FG and β-actin genes was reciprocal. Conclusion: FG expression is transiently suppressed when cytoskeleton gene expression is enhanced at the early phase of liver regeneration.",
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AU - Sasaki, Shunsuke

AU - Oka, Takahiko

AU - Takayama, Niro

AU - Shiraha, Hidenori

AU - Ujike, Kozo

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AU - Hanafusa, Tadashi

AU - Yumoto, Yasuhiro

AU - Tsuji, Takao

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