Receptor Sorting within Endosomal Trafficking Pathway is Facilitated by Dynamic Actin Filaments

Emiko Ohashi, Kenji Tanabe, Yuji Henmi, Kumi Mesaki, Yuka Kobayashi, Kohji Takei

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Early endosomes (EEs) are known to be a sorting station for internalized molecules destined for degradation, recycling, or other intracellular organelles. Segregation is an essential step in such sorting, but the molecular mechanism of this process remains to be elucidated. Here, we show that actin is required for efficient recycling and endosomal maturation by producing a motile force. Perturbation of actin dynamics by drugs induced a few enlarged EEs containing several degradative vacuoles and also interfered with their transporting ability. Actin repolymerization induced by washout of the drug caused the vacuoles to dissociate and individually translocate toward the perinuclear region. We further elucidated that cortactin, an actin-nucleating factor, was required for transporting contents from within EEs. Actin filaments regulated by cortactin may provide a motile force for efficient sorting within early endosomes. These data suggest that actin filaments coordinate with microtubules to mediate segregation in EEs.

Original languageEnglish
Article numbere19942
JournalPLoS One
Volume6
Issue number5
DOIs
Publication statusPublished - 2011

Fingerprint

endosomes
Endosomes
microfilaments
Actin Cytoskeleton
Sorting
sorting
Actins
actin
receptors
Cortactin
Recycling
Vacuoles
recycling
vacuoles
drugs
Microtubules
Pharmaceutical Preparations
Organelles
microtubules
organelles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Receptor Sorting within Endosomal Trafficking Pathway is Facilitated by Dynamic Actin Filaments. / Ohashi, Emiko; Tanabe, Kenji; Henmi, Yuji; Mesaki, Kumi; Kobayashi, Yuka; Takei, Kohji.

In: PLoS One, Vol. 6, No. 5, e19942, 2011.

Research output: Contribution to journalArticle

Ohashi, Emiko ; Tanabe, Kenji ; Henmi, Yuji ; Mesaki, Kumi ; Kobayashi, Yuka ; Takei, Kohji. / Receptor Sorting within Endosomal Trafficking Pathway is Facilitated by Dynamic Actin Filaments. In: PLoS One. 2011 ; Vol. 6, No. 5.
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