Receptor protein tyrosine kinase DDR is up-regulated by p53 protein

Shirou Sakuma, Hideyuki Saya, Mitsuhiro Tada, Mitsuyoshi Nakao, Toshiyoshi Fujiwara, Jack A. Roth, Yutaka Sawamura, Yumiko Shinohe, Abe Hiroshi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have previously reported on radiation-induction of ptk-3 in rat astr ocyte culture [Sakuma et al. (1995) Radiat. Res. 143, 1-7]. Ptk-3 was considered to be a rat version of human DDR (discoidin domain receptor). We cloned and analyzed genomic DNA of the DDR and its promoter region. We discovered that the promoter region contained a consensus sequence of the p53 tumor suppressor binding site. Adenovirus-mediated p53 transfection induced a high level of DDR mRNA in SAOS2 human osteosarcoma cells. These results indicate that DDR is up-regulated by the p53 protein.

Original languageEnglish
Pages (from-to)165-169
Number of pages5
JournalFEBS Letters
Volume398
Issue number2-3
DOIs
Publication statusPublished - Dec 2 1996

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Receptor Protein-Tyrosine Kinases
Genetic Promoter Regions
Rats
Tumors
Proteins
Binding Sites
Radiation
Messenger RNA
DNA
Consensus Sequence
Osteosarcoma
Adenoviridae
Transfection
Discoidin Domain Receptors
Neoplasms

Keywords

  • Genomic DNA structure
  • Human DDR gene
  • p53 regulatory element

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Sakuma, S., Saya, H., Tada, M., Nakao, M., Fujiwara, T., Roth, J. A., ... Hiroshi, A. (1996). Receptor protein tyrosine kinase DDR is up-regulated by p53 protein. FEBS Letters, 398(2-3), 165-169. https://doi.org/10.1016/S0014-5793(96)01234-3

Receptor protein tyrosine kinase DDR is up-regulated by p53 protein. / Sakuma, Shirou; Saya, Hideyuki; Tada, Mitsuhiro; Nakao, Mitsuyoshi; Fujiwara, Toshiyoshi; Roth, Jack A.; Sawamura, Yutaka; Shinohe, Yumiko; Hiroshi, Abe.

In: FEBS Letters, Vol. 398, No. 2-3, 02.12.1996, p. 165-169.

Research output: Contribution to journalArticle

Sakuma, S, Saya, H, Tada, M, Nakao, M, Fujiwara, T, Roth, JA, Sawamura, Y, Shinohe, Y & Hiroshi, A 1996, 'Receptor protein tyrosine kinase DDR is up-regulated by p53 protein', FEBS Letters, vol. 398, no. 2-3, pp. 165-169. https://doi.org/10.1016/S0014-5793(96)01234-3
Sakuma, Shirou ; Saya, Hideyuki ; Tada, Mitsuhiro ; Nakao, Mitsuyoshi ; Fujiwara, Toshiyoshi ; Roth, Jack A. ; Sawamura, Yutaka ; Shinohe, Yumiko ; Hiroshi, Abe. / Receptor protein tyrosine kinase DDR is up-regulated by p53 protein. In: FEBS Letters. 1996 ; Vol. 398, No. 2-3. pp. 165-169.
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