Recent advances in controlled immobilization of proteins onto the surface of the solid substrate and its possible application to proteomics

Kazuhiro Nakanishi, Takaharu Sakiyama, Yoichi Kumada, Koreyoshi Imamura, Hiroyuki Imanaka

Research output: Contribution to journalReview article

77 Citations (Scopus)

Abstract

Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.

Original languageEnglish
Pages (from-to)161-175
Number of pages15
JournalCurrent Proteomics
Volume5
Issue number3
DOIs
Publication statusPublished - Oct 1 2008

Keywords

  • Affinity peptide
  • Controlled immobilization
  • ELISA
  • Protein-protein interaction
  • Proteome analysis
  • Site-directed immobilization

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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