Abstract
Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.
Original language | English |
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Pages (from-to) | 161-175 |
Number of pages | 15 |
Journal | Current Proteomics |
Volume | 5 |
Issue number | 3 |
DOIs | |
Publication status | Published - Oct 1 2008 |
Keywords
- Affinity peptide
- Controlled immobilization
- ELISA
- Protein-protein interaction
- Proteome analysis
- Site-directed immobilization
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology