Real-time monitoring of tumor progression and drug responses in a preclinical mouse model of prostate cancer

Peng Xu, Naijin Xu, Kai Guo, Abai Xu, Fumiaki Takenaka, Eiji Matsuura, Chunxiao Liu, Hiromi Kumon, Peng Huang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Monitoring disease progression through imaging is playing an increasingly important role in the treatment of prostate cancer. Here, we report that primary mouse prostate cancer cell lines stably expressing luciferase and tumor biomarkers can be monitored through bioluminescence imaging along with assays of serum biomarkers and immune function. Tumorigenesis in immunocompetent C57BL/6 mice can be monitored in by collecting samples from the dorsal flank, dorsolateral prostate, and tail vein to obtain real-time subcutaneous, orthotopic, and metastasis indicators, respectively. We used this technique to confirm the therapeutic effect of immune checkpoint blockade. Our findings suggest the presented indicators are ideally suited for real-time tracking of drug responses, tumor progression and immune function.

Original languageEnglish
Pages (from-to)33025-33034
Number of pages10
JournalOncotarget
Volume7
Issue number22
DOIs
Publication statusPublished - May 31 2016

Keywords

  • Bioluminescence imaging
  • Immunotherapy
  • Preclinical model
  • Prostate cancer
  • Prostate specific antigen

ASJC Scopus subject areas

  • Oncology

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