Real-time imaging of mast cell degranulation in vitro and in vivo

Kayo Horiguchi, Soichiro Yoshikawa, Asuka Saito, Salma Haddad, Takuya Ohta, Kensuke Miyake, Yoshinori Yamanishi, Hajime Karasuyama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mast cells undergo degranulation in response to various stimuli and rapidly release pre-formed mediators present in secretory granules, leading to immediate-type allergic reactions. Mast cell degranulation is commonly detected and quantified in vitro by measuring histamine or β-hexosaminidase released to culture medium. However, this type of assay cannot monitor degranulation of individual cells in real time, and it is not suitable for in vivo detection of degranulation. At the aim of real time imaging of mast cell degranulation at single cell level, we here developed a fluorescent protein-based indicator of degranulation, designated immuno-pHluorin (impH). When expressed in mast cells, impH is located in the membrane of secretory granules and non-fluorescent under homeostatic conditions while it turns fluorescent following degranulation, due to the pH change inside of granules during exocytosis. impH enabled us to detect polarized degranulation within one single cell when mast cells were stimulated via the small area of cell surface. Transplantation of impH-expressing mast cells into mast cell-deficient mice demonstrated that impH could function as a real-time indicator of degranulation in vivo. Thus, impH is a useful tool for imaging of mast cell activation and degranulation in vitro and in vivo, and may be applied for screening of reagents regulating mast cell degranulation.

Original languageEnglish
Pages (from-to)517-522
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume479
Issue number3
DOIs
Publication statusPublished - Oct 21 2016
Externally publishedYes

Fingerprint

Cell Degranulation
Mast Cells
Imaging techniques
Secretory Vesicles
Hexosaminidases
Histamine
Culture Media
PHluorin
Assays
Screening
Chemical activation
Exocytosis
Membranes
Hypersensitivity
Transplantation
Proteins

Keywords

  • Degranulation
  • Exocytosis
  • Imaging
  • Mast cell
  • pH sensor
  • VAMP-8

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Real-time imaging of mast cell degranulation in vitro and in vivo. / Horiguchi, Kayo; Yoshikawa, Soichiro; Saito, Asuka; Haddad, Salma; Ohta, Takuya; Miyake, Kensuke; Yamanishi, Yoshinori; Karasuyama, Hajime.

In: Biochemical and Biophysical Research Communications, Vol. 479, No. 3, 21.10.2016, p. 517-522.

Research output: Contribution to journalArticle

Horiguchi, K, Yoshikawa, S, Saito, A, Haddad, S, Ohta, T, Miyake, K, Yamanishi, Y & Karasuyama, H 2016, 'Real-time imaging of mast cell degranulation in vitro and in vivo', Biochemical and Biophysical Research Communications, vol. 479, no. 3, pp. 517-522. https://doi.org/10.1016/j.bbrc.2016.09.100
Horiguchi, Kayo ; Yoshikawa, Soichiro ; Saito, Asuka ; Haddad, Salma ; Ohta, Takuya ; Miyake, Kensuke ; Yamanishi, Yoshinori ; Karasuyama, Hajime. / Real-time imaging of mast cell degranulation in vitro and in vivo. In: Biochemical and Biophysical Research Communications. 2016 ; Vol. 479, No. 3. pp. 517-522.
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