Abstract
Due to the high corrosion resistance and strength to density ratio titanium is widely used in industry, and also in a gamut of medical applications. Here we report for the first time on our development of a titanium passivation layer sensor that makes use of surface plasmon resonance (SPR). The deposited titanium metal layer on the sensor was passivated in air, similarly to titanium medical devices. Our "Ti-SPR sensor" enables analysis of biomolecule interactions with the passivated surface of titanium in real time. As a proof of concept, corrosion of a titanium passivation layer exposed to acid was monitored in real time. The Ti-SPR sensor can also accurately measure the time-dependence of protein adsorption onto the titanium passivation layer at sub-nanogram per square millimeter accuracy. Besides such SPR analyses, SPR imaging (SPRI) enables real time assessment of chemical surface processes that occur simultaneously at "multiple independent spots" on the Ti-SPR sensor, such as acid corrosion or adhesion of cells. Our Ti-SPR sensor will therefore be very useful to study titanium corrosion phenomena and biomolecular titanium-surface interactions with application in a broad range of industrial and biomedical fields.
| Original language | English |
|---|---|
| Pages (from-to) | 1260-1266 |
| Number of pages | 7 |
| Journal | Acta Biomaterialia |
| Volume | 8 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2012 |
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Keywords
- Biosensor
- Cell adhesion
- Protein adsorption
- Surface plasmon resonance
- Titanium passivation layer
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- Biotechnology
- Biochemistry
- Molecular Biology
Cite this
Real time assessment of surface interactions with a titanium passivation layer by surface plasmon resonance. / Hirata, Isao; Yoshida, Yasuhiro; Nagaoka, Noriyuki; Hiasa, Kyou; Abe, Yasuhiko; Maekawa, Kenji; Kuboki, Takuo; Akagawa, Yasumasa; Suzuki, Kazuomi; Meerbeek, Bart Van; Messersmith, Phillip B.; Okazaki, Masayuki.
In: Acta Biomaterialia, Vol. 8, No. 3, 03.2012, p. 1260-1266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Real time assessment of surface interactions with a titanium passivation layer by surface plasmon resonance
AU - Hirata, Isao
AU - Yoshida, Yasuhiro
AU - Nagaoka, Noriyuki
AU - Hiasa, Kyou
AU - Abe, Yasuhiko
AU - Maekawa, Kenji
AU - Kuboki, Takuo
AU - Akagawa, Yasumasa
AU - Suzuki, Kazuomi
AU - Meerbeek, Bart Van
AU - Messersmith, Phillip B.
AU - Okazaki, Masayuki
PY - 2012/3
Y1 - 2012/3
N2 - Due to the high corrosion resistance and strength to density ratio titanium is widely used in industry, and also in a gamut of medical applications. Here we report for the first time on our development of a titanium passivation layer sensor that makes use of surface plasmon resonance (SPR). The deposited titanium metal layer on the sensor was passivated in air, similarly to titanium medical devices. Our "Ti-SPR sensor" enables analysis of biomolecule interactions with the passivated surface of titanium in real time. As a proof of concept, corrosion of a titanium passivation layer exposed to acid was monitored in real time. The Ti-SPR sensor can also accurately measure the time-dependence of protein adsorption onto the titanium passivation layer at sub-nanogram per square millimeter accuracy. Besides such SPR analyses, SPR imaging (SPRI) enables real time assessment of chemical surface processes that occur simultaneously at "multiple independent spots" on the Ti-SPR sensor, such as acid corrosion or adhesion of cells. Our Ti-SPR sensor will therefore be very useful to study titanium corrosion phenomena and biomolecular titanium-surface interactions with application in a broad range of industrial and biomedical fields.
AB - Due to the high corrosion resistance and strength to density ratio titanium is widely used in industry, and also in a gamut of medical applications. Here we report for the first time on our development of a titanium passivation layer sensor that makes use of surface plasmon resonance (SPR). The deposited titanium metal layer on the sensor was passivated in air, similarly to titanium medical devices. Our "Ti-SPR sensor" enables analysis of biomolecule interactions with the passivated surface of titanium in real time. As a proof of concept, corrosion of a titanium passivation layer exposed to acid was monitored in real time. The Ti-SPR sensor can also accurately measure the time-dependence of protein adsorption onto the titanium passivation layer at sub-nanogram per square millimeter accuracy. Besides such SPR analyses, SPR imaging (SPRI) enables real time assessment of chemical surface processes that occur simultaneously at "multiple independent spots" on the Ti-SPR sensor, such as acid corrosion or adhesion of cells. Our Ti-SPR sensor will therefore be very useful to study titanium corrosion phenomena and biomolecular titanium-surface interactions with application in a broad range of industrial and biomedical fields.
KW - Biosensor
KW - Cell adhesion
KW - Protein adsorption
KW - Surface plasmon resonance
KW - Titanium passivation layer
UR - http://www.scopus.com/inward/record.url?scp=84856537252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84856537252&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2011.11.025
DO - 10.1016/j.actbio.2011.11.025
M3 - Article
C2 - 22154862
AN - SCOPUS:84856537252
VL - 8
SP - 1260
EP - 1266
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
IS - 3
ER -