Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol

Toshio Araki, Tomoko Nakatsuka, Fuminao Kobayashi, Emi Watanabe-Ishimaru, Hirokazu Sanada, Takashi Tamura, Kenji Inagaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purified recombinant sorbose dehydrogenase from Sinorhizobium sp. 97507 exhibited high reactivity for 1,5-anhydro-D-glucitol (1,5-AG) and L-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (kcat/Km) for L-sorbose and 1,5-AG is 1.8 × 102 and 1.5 × 102 s-1·M-1, respectively.

Original languageEnglish
Pages (from-to)1130-1132
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number7
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • 1,5-anhydro-D-glucitol
  • Sinorhizobium sp.
  • Sorbose dehydrogenase

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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