Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol

Toshio Araki; Tomoko Nakatsuka; Fuminao Kobayashi; Emi Watanabe-Ishimaru; Hirokazu Sanada; Takashi Tamura; Kenji Inagaki

doi:10.1080/09168451.2015.1012148

Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol

Toshio Araki, Tomoko Nakatsuka, Fuminao Kobayashi, Emi Watanabe-Ishimaru, Hirokazu Sanada, Takashi Tamura, Kenji Inagaki

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Purified recombinant sorbose dehydrogenase from Sinorhizobium sp. 97507 exhibited high reactivity for 1,5-anhydro-D-glucitol (1,5-AG) and L-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (k_cat/K_m) for L-sorbose and 1,5-AG is 1.8 × 10² and 1.5 × 10² s^-1·M^-1, respectively.

Original language	English
Pages (from-to)	1130-1132
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	79
Issue number	7
DOIs	https://doi.org/10.1080/09168451.2015.1012148
Publication status	Published - 2015

Keywords

1,5-anhydro-D-glucitol
Sinorhizobium sp.
Sorbose dehydrogenase

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol. / Araki, Toshio; Nakatsuka, Tomoko; Kobayashi, Fuminao; Watanabe-Ishimaru, Emi; Sanada, Hirokazu; Tamura, Takashi ; Inagaki, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 7, 2015, p. 1130-1132.

Research output: Contribution to journal › Article › peer-review

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abstract = "Purified recombinant sorbose dehydrogenase from Sinorhizobium sp. 97507 exhibited high reactivity for 1,5-anhydro-D-glucitol (1,5-AG) and L-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (kcat/Km) for L-sorbose and 1,5-AG is 1.8 × 102 and 1.5 × 102 s-1·M-1, respectively.",

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AU - Watanabe-Ishimaru, Emi

AU - Sanada, Hirokazu

AU - Tamura, Takashi

AU - Inagaki, Kenji

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KW - Sinorhizobium sp.

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