Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol

Toshio Araki, Tomoko Nakatsuka, Fuminao Kobayashi, Emi Watanabe-Ishimaru, Hirokazu Sanada, Takashi Tamura, Kenji Inagaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purified recombinant sorbose dehydrogenase from Sinorhizobium sp. 97507 exhibited high reactivity for 1,5-anhydro-D-glucitol (1,5-AG) and L-sorbose, but little activity for the other sugars or sugar alcohols tested. Kinetic analysis revealed that its catalytic efficiency (kcat/Km) for L-sorbose and 1,5-AG is 1.8 × 102 and 1.5 × 102 s-1·M-1, respectively.

Original languageEnglish
Pages (from-to)1130-1132
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume79
Issue number7
DOIs
Publication statusPublished - 2015

Fingerprint

Sinorhizobium
Sorbose
Sugar Alcohols
Sugars
Kinetics
sorbose dehydrogenase
1,5-anhydroglucitol

Keywords

  • 1,5-anhydro-D-glucitol
  • Sinorhizobium sp.
  • Sorbose dehydrogenase

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol. / Araki, Toshio; Nakatsuka, Tomoko; Kobayashi, Fuminao; Watanabe-Ishimaru, Emi; Sanada, Hirokazu; Tamura, Takashi; Inagaki, Kenji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 79, No. 7, 2015, p. 1130-1132.

Research output: Contribution to journalArticle

Araki, Toshio ; Nakatsuka, Tomoko ; Kobayashi, Fuminao ; Watanabe-Ishimaru, Emi ; Sanada, Hirokazu ; Tamura, Takashi ; Inagaki, Kenji. / Reactivity of sorbose dehydrogenase from Sinorhizobium sp. 97507 for 1,5-anhydro-D-glucitol. In: Bioscience, Biotechnology and Biochemistry. 2015 ; Vol. 79, No. 7. pp. 1130-1132.
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