Reactive carbonyl species mediate methyl jasmonate-induced stomatal closure

Md Moshiul Islam, Wenxiu Ye, Fahmida Akter, Mohammad Saidur Rhaman, Daiki Matsushima, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md Sanaullah Biswas, Junichi Mano, Yoshiyuki Murata

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Production of reactive oxygen species (ROS) is a key signal event for methyl jasmonate (MeJA)- and abscisic acid (ABA)- induced stomatal closure. We recently showed that reactive carbonyl species (RCS) stimulates stomatal closure as an intermediate downstream of hydrogen peroxide (H2O2) production in the ABA signaling pathway in guard cells of Nicotiana tabacum and Arabidopsis thaliana. In this study, we examined whether RCS functions as an intermediate downstream of H2O2 production in MeJA signaling in guard cells using transgenic tobacco plants overexpressing A. thaliana 2-alkenal reductase (n-alkanal NAD(P) -2-alkenal + NAD(P)H+H + ) (AER-OE tobacco) and Arabidopsis plants. The stomatal closure induced by MeJA was impaired in the AER-OE tobacco and was inhibited by RCS scavengers, carnosine and pyridoxamine, in the wild-type (WT) tobacco plants and Arabidopsis plants. Application of MeJA significantly induced the accumulation of RCS, including acrolein and 4-hydroxy-(E)-2-nonenal, in the WT tobacco but not in the AER-OE plants. Application of MeJA induced H2O2 production in the WT tobacco and the AER-OE plants and the H2O2 production was not inhibited by the RCS scavengers. These results suggest that RCS functions as an intermediate downstream of ROS production in MeJA signaling and in ABA signaling in guard cells.

Original languageEnglish
Pages (from-to)1788-1797
Number of pages10
JournalPlant and Cell Physiology
Volume61
Issue number10
DOIs
Publication statusPublished - 2020

Keywords

  • Cytosolic Ca2+ elevation
  • Methyl jasmonate
  • Reactive carbonyl species
  • Reactive oxygen species
  • Stomatal closure

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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