TY - JOUR
T1 - Reactive carbonyl species mediate ABA signaling in guard cells
AU - Islam, Md Moshiul
AU - Ye, Wenxiu
AU - Matsushima, Daiki
AU - Munemasa, Shintaro
AU - Okuma, Eiji
AU - Nakamura, Yoshimasa
AU - Biswas, Md Sanaullah
AU - Mano, Jun'ichi
AU - Murata, Yoshiyuki
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science [Grants-in-Aid for Fellows (to W.Y.) and by Grants-in Aid for Scientific Research (C) No. 26440149 (to J.M.)].
Publisher Copyright:
© The Authors 2016.
PY - 2016/12
Y1 - 2016/12
N2 - Drought is responsible for a massive reduction in crop yields. In response to drought, plants synthesize the hormone ABA, which induces stomatal closure, thus reducing water loss. In guard cells, ABA triggers production of reactive oxygen species (ROS), which is mediated by NAD(P)H oxidases. The production of ROS is a key factor for ABA-induced stomatal closure, but it remains to be clarified how the production of ROS is transduced into downstream signaling components in guard cells. We investigated roles of reactive carbonyl species (RCS) in ABA-induced stomatal closure using transgenic tobacco (Nicotiana tabacum) overexpressing Arabidopsis 2-alkenal reductase (AER-OE), which scavenges RCS. ABA and hydrogen peroxide (H2O2) induced accumulation of RCS including acrolein and 4-hydroxy-(E)-2-nonenal in wild-type tobacco but not in AER-OE. Stomatal closure and RCS accumulation in response to ABA and H2O2 were inhibited in AER-OE unlike in the wild type, while ABA-induced H2O2 production in guard cells was observed in AER-OE as well as in the wild type. Moreover, ABA inhibited inward-rectifying K+ channels in wild-type guard cells but not in AER-OE guard cells. These results suggest that RCS is involved in ABAinduced stomatal closure and functions downstream of H2O2 production in the ABA signaling pathway in guard cells.
AB - Drought is responsible for a massive reduction in crop yields. In response to drought, plants synthesize the hormone ABA, which induces stomatal closure, thus reducing water loss. In guard cells, ABA triggers production of reactive oxygen species (ROS), which is mediated by NAD(P)H oxidases. The production of ROS is a key factor for ABA-induced stomatal closure, but it remains to be clarified how the production of ROS is transduced into downstream signaling components in guard cells. We investigated roles of reactive carbonyl species (RCS) in ABA-induced stomatal closure using transgenic tobacco (Nicotiana tabacum) overexpressing Arabidopsis 2-alkenal reductase (AER-OE), which scavenges RCS. ABA and hydrogen peroxide (H2O2) induced accumulation of RCS including acrolein and 4-hydroxy-(E)-2-nonenal in wild-type tobacco but not in AER-OE. Stomatal closure and RCS accumulation in response to ABA and H2O2 were inhibited in AER-OE unlike in the wild type, while ABA-induced H2O2 production in guard cells was observed in AER-OE as well as in the wild type. Moreover, ABA inhibited inward-rectifying K+ channels in wild-type guard cells but not in AER-OE guard cells. These results suggest that RCS is involved in ABAinduced stomatal closure and functions downstream of H2O2 production in the ABA signaling pathway in guard cells.
KW - ABA
KW - Reactive carbonyl species
KW - Reactive oxygen species
KW - Stomatal closure
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U2 - 10.1093/pcp/pcw166
DO - 10.1093/pcp/pcw166
M3 - Article
C2 - 27838658
AN - SCOPUS:85014243212
VL - 57
SP - 2552
EP - 2563
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 12
ER -