Reactive Carbonyl Species Function as Signal Mediators Downstream of H2O2 Production and Regulate [Ca2+]cyt Elevation in ABA Signal Pathway in Arabidopsis Guard Cells

Md Moshiul Islam, Wenxiu Ye, Daiki Matsushima, Mohammad Saidur Rhaman, Shintaro Munemasa, Eiji Okuma, Yoshimasa Nakamura, Md Sanaullah Biswas, Jun'ichi Mano, Yoshiyuki Murata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have demonstrated that reactive carbonyl species (RCS) function as an intermediate downstream of hydrogen peroxide (H2O2) production in abscisic acid (ABA) signaling for stomatal closure in guard cells using transgenic tobacco plants overexpressing alkenal reductase. We investigated the conversion of the RCS production into downstream signaling events in the guard cells. Both ABA and H2O2 induced production of the RCS, such as acrolein and 4-hydroxy-(E)-2-nonenal (HNE), in epidermal tissues of wild-type Arabidopsis thaliana plants. Application of the RCS scavengers, carnosine and pyridoxamine, did not affect the ABA-induced H2O2 production but inhibited the ABA- and H2O2-induced stomatal closure. Both acrolein and HNE induced stomatal closure in a plasma membrane NAD(P)H oxidase mutant atrbohD atrbohF as well as in the wild type, but not in a calcium-dependent kinase mutant cpk6. Acrolein activated plasma membrane Ca2+-permeable cation channels, triggered cytosolic free Ca2+ concentration ([Ca2+]cyt) elevation, and induced stomatal closure accompanied by depletion of glutathione in the guard cells. These results suggest that RCS production is a signaling event between the ROS production and [Ca2+]cyt elevation during guard cell ABA signaling.

Original languageEnglish
Pages (from-to)1146-1159
Number of pages14
JournalPlant & cell physiology
Volume60
Issue number5
DOIs
Publication statusPublished - May 1 2019

Fingerprint

Abscisic Acid
guard cells
Arabidopsis
signal transduction
abscisic acid
Signal Transduction
acrolein
Acrolein
calcium
plasma membrane
Pyridoxamine
Cell Membrane
pyridoxamine
Carnosine
carnosine
mutants
NADPH Oxidase
Genetically Modified Plants
Hydrogen Peroxide
Tobacco

Keywords

  • Abscisic acid
  • Arabidopsis
  • Cytosolic calcium
  • Reactive carbonyl species
  • Reactive oxygen species
  • Stomatal closure

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Reactive Carbonyl Species Function as Signal Mediators Downstream of H2O2 Production and Regulate [Ca2+]cyt Elevation in ABA Signal Pathway in Arabidopsis Guard Cells. / Islam, Md Moshiul; Ye, Wenxiu; Matsushima, Daiki; Rhaman, Mohammad Saidur; Munemasa, Shintaro; Okuma, Eiji; Nakamura, Yoshimasa; Biswas, Md Sanaullah; Mano, Jun'ichi; Murata, Yoshiyuki.

In: Plant & cell physiology, Vol. 60, No. 5, 01.05.2019, p. 1146-1159.

Research output: Contribution to journalArticle

Islam, Md Moshiul ; Ye, Wenxiu ; Matsushima, Daiki ; Rhaman, Mohammad Saidur ; Munemasa, Shintaro ; Okuma, Eiji ; Nakamura, Yoshimasa ; Biswas, Md Sanaullah ; Mano, Jun'ichi ; Murata, Yoshiyuki. / Reactive Carbonyl Species Function as Signal Mediators Downstream of H2O2 Production and Regulate [Ca2+]cyt Elevation in ABA Signal Pathway in Arabidopsis Guard Cells. In: Plant & cell physiology. 2019 ; Vol. 60, No. 5. pp. 1146-1159.
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AU - Rhaman, Mohammad Saidur

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AU - Okuma, Eiji

AU - Nakamura, Yoshimasa

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AU - Murata, Yoshiyuki

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