Abstract
An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4alpha2 (HNF4alpha2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4alpha2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, alpha1-antitrypsin, and HNF1alpha were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.
| Original language | English |
|---|---|
| Pages (from-to) | 481-487 |
| Number of pages | 7 |
| Journal | International Journal of Molecular Medicine |
| Volume | 8 |
| Issue number | 5 |
| Publication status | Published - Nov 2001 |
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ASJC Scopus subject areas
- Genetics
Cite this
Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4alpha2 gene. / Inoue, Y.; Miyazaki, M.; Tsuji, T.; Sakaguchi, Masakiyo; Fukaya, K.; Huh, N. H.; Namba, M.
In: International Journal of Molecular Medicine, Vol. 8, No. 5, 11.2001, p. 481-487.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4alpha2 gene.
AU - Inoue, Y.
AU - Miyazaki, M.
AU - Tsuji, T.
AU - Sakaguchi, Masakiyo
AU - Fukaya, K.
AU - Huh, N. H.
AU - Namba, M.
PY - 2001/11
Y1 - 2001/11
N2 - An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4alpha2 (HNF4alpha2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4alpha2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, alpha1-antitrypsin, and HNF1alpha were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.
AB - An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4alpha2 (HNF4alpha2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4alpha2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, alpha1-antitrypsin, and HNF1alpha were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.
UR - http://www.scopus.com/inward/record.url?scp=0035514239&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035514239&partnerID=8YFLogxK
M3 - Article
C2 - 11605014
AN - SCOPUS:0035514239
VL - 8
SP - 481
EP - 487
JO - International Journal of Molecular Medicine
JF - International Journal of Molecular Medicine
SN - 1107-3756
IS - 5
ER -