Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4alpha2 gene.

Y. Inoue, M. Miyazaki, T. Tsuji, Masakiyo Sakaguchi, K. Fukaya, N. H. Huh, M. Namba

Research output: Contribution to journalArticle

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Abstract

An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4alpha2 (HNF4alpha2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4alpha2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, alpha1-antitrypsin, and HNF1alpha were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.

Original languageEnglish
Pages (from-to)481-487
Number of pages7
JournalInternational Journal of Molecular Medicine
Volume8
Issue number5
Publication statusPublished - Nov 2001

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Hepatocyte Nuclear Factors
Hepatocytes
Gene Expression
Cell Line
Liver
Genes
Apolipoprotein C-II
Factor X
Cytochrome P-450 CYP1A1
Viral Tumor Antigens
Apolipoprotein A-I
Cell Nucleus
Transfection
Albumins
DNA
Enzymes

ASJC Scopus subject areas

  • Genetics

Cite this

Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4alpha2 gene. / Inoue, Y.; Miyazaki, M.; Tsuji, T.; Sakaguchi, Masakiyo; Fukaya, K.; Huh, N. H.; Namba, M.

In: International Journal of Molecular Medicine, Vol. 8, No. 5, 11.2001, p. 481-487.

Research output: Contribution to journalArticle

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AU - Huh, N. H.

AU - Namba, M.

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