Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives

Annalisa Mazzoni, David H. Pashley, Yoshihiro Nishitani, Lorenzo Breschi, Ferdinando Mannello, Leo Tjäderhane, Manuel Toledano, Edna L. Pashley, Franklin R. Tay

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

Auto-degradation of collagen matrices occurs in resin-infiltrated dentine by the slow action of host-derived matrix metalloproteinases. As phosphoric acid-etching inactivates these endogenous enzymes, it is puzzling how hybrid layers created by simplified etch-and-rinse adhesives can degrade in vivo. This study tested the null hypothesis that there are no differences in the relative proteolytic activities of mineralised dentine, acid-etched dentine, and etch-and-rinse adhesivetreated acid-etched dentine. Powdered dentine prepared from extracted human teeth was treated with 17% EDTA, 10% phosphoric acid, or with five simplified etch-and-rinse adhesives that were applied to 10% phosphoric acid-etched dentine. The gelatinolytic activity of the dentine powder was assayed using fluorescein-labelled gelatine. TEM examination of the air-dried, treated dentine powder was performed to confirm the presence of remnant mineralised dentine after acid-etching. 17% EDTA significantly reduced the relative proteolytic activity (73.2%) of the untreated mineralised dentine powder (control), while 10% phosphoric acid-etched dentine exhibited the highest reduction (98.1%). Treating the acid-etched dentine powder with any of the five simplified etch-and-rinse adhesives resulted in the reactivation of the proteolytic activity, with a significant negative linear correlation ( P <0.05) between the increases in fluorescence and the corresponding pH values of the adhesives. It is concluded that simplified etch-and-rinse adhesives can reactivate endogenous enzymatic activities in dentine that are previously inactivated by phosphoric acid-etching. The amount of enzyme reactivated may even exceed the original quantity present in untreated mineralised dentine. This provides an explanation for the degradation of hybrid layers after acid-etched dentine matrices are infiltrated with these adhesives.

Original languageEnglish
Pages (from-to)4470-4476
Number of pages7
JournalBiomaterials
Volume27
Issue number25
DOIs
Publication statusPublished - Sep 2006

Fingerprint

Phosphoric acid
Dentin
Adhesives
Powders
Acids
Etching
Ethylenediaminetetraacetic acid
Edetic Acid
Enzymes
Degradation
Fluorescein
Matrix Metalloproteinases
Collagen
phosphoric acid
Resins
Fluorescence
Transmission electron microscopy
Air
Tooth

Keywords

  • Acid-etched dentine
  • Collagen degradation
  • Etch-and-rinse adhesives
  • Fluorescence
  • Fluorometric enzymatic assay
  • Proteolytic activity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Mazzoni, A., Pashley, D. H., Nishitani, Y., Breschi, L., Mannello, F., Tjäderhane, L., ... Tay, F. R. (2006). Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials, 27(25), 4470-4476. https://doi.org/10.1016/j.biomaterials.2006.01.040

Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. / Mazzoni, Annalisa; Pashley, David H.; Nishitani, Yoshihiro; Breschi, Lorenzo; Mannello, Ferdinando; Tjäderhane, Leo; Toledano, Manuel; Pashley, Edna L.; Tay, Franklin R.

In: Biomaterials, Vol. 27, No. 25, 09.2006, p. 4470-4476.

Research output: Contribution to journalArticle

Mazzoni, A, Pashley, DH, Nishitani, Y, Breschi, L, Mannello, F, Tjäderhane, L, Toledano, M, Pashley, EL & Tay, FR 2006, 'Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives', Biomaterials, vol. 27, no. 25, pp. 4470-4476. https://doi.org/10.1016/j.biomaterials.2006.01.040
Mazzoni, Annalisa ; Pashley, David H. ; Nishitani, Yoshihiro ; Breschi, Lorenzo ; Mannello, Ferdinando ; Tjäderhane, Leo ; Toledano, Manuel ; Pashley, Edna L. ; Tay, Franklin R. / Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. In: Biomaterials. 2006 ; Vol. 27, No. 25. pp. 4470-4476.
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abstract = "Auto-degradation of collagen matrices occurs in resin-infiltrated dentine by the slow action of host-derived matrix metalloproteinases. As phosphoric acid-etching inactivates these endogenous enzymes, it is puzzling how hybrid layers created by simplified etch-and-rinse adhesives can degrade in vivo. This study tested the null hypothesis that there are no differences in the relative proteolytic activities of mineralised dentine, acid-etched dentine, and etch-and-rinse adhesivetreated acid-etched dentine. Powdered dentine prepared from extracted human teeth was treated with 17{\%} EDTA, 10{\%} phosphoric acid, or with five simplified etch-and-rinse adhesives that were applied to 10{\%} phosphoric acid-etched dentine. The gelatinolytic activity of the dentine powder was assayed using fluorescein-labelled gelatine. TEM examination of the air-dried, treated dentine powder was performed to confirm the presence of remnant mineralised dentine after acid-etching. 17{\%} EDTA significantly reduced the relative proteolytic activity (73.2{\%}) of the untreated mineralised dentine powder (control), while 10{\%} phosphoric acid-etched dentine exhibited the highest reduction (98.1{\%}). Treating the acid-etched dentine powder with any of the five simplified etch-and-rinse adhesives resulted in the reactivation of the proteolytic activity, with a significant negative linear correlation ( P <0.05) between the increases in fluorescence and the corresponding pH values of the adhesives. It is concluded that simplified etch-and-rinse adhesives can reactivate endogenous enzymatic activities in dentine that are previously inactivated by phosphoric acid-etching. The amount of enzyme reactivated may even exceed the original quantity present in untreated mineralised dentine. This provides an explanation for the degradation of hybrid layers after acid-etched dentine matrices are infiltrated with these adhesives.",
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