Re-mineralizing dentin using an experimental tricalcium silicate cement with biomimetic analogs

Xin Li, Jan De Munck, Kumiko Yoshihara, Mariano Pedano, Kirsten Van Landuyt, Zhi Chen, Bart Van Meerbeek

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives To characterize the re-mineralization potential of an experimental zirconium oxide (ZrO2) containing tricalcium silicate (TCS) cement, TCS 50, with the incorporation of biomimetic analogs at demineralized dentin. Methods Class-I cavities were prepared in non- carious human third molars. The dentin cavities were demineralized using a pH-cycling protocol, involving 50 cyclic immersions in pH-4.8 and pH-7 baths for 0.5 h and 2.5 h, successively. The cavities were filled with TCS 50 with/without biomimetic analogs (3% polyacrylic acid, 8% sodium trimetaphosphate) being added to the mixed TCS 50 cement prior to application. The commercial hCSCs Biodentine (Septodont) and ProRoot MTA (Dentsply Sirona) served as controls. After 1 and 6 weeks storage in simulated body fluid (SBF), the polished specimen cross-sections were chemically characterized using a field-emission-gun Electron Probe Micro-Analysis (Feg- EPMA). Results EPMA line-scans and elemental mappings confirmed early re-mineralization induced by TCS 50 at 1 week. When biomimetic analogs were added to TCS 50, re-mineralization was more efficient after 6 weeks; the relative depth and intensity of re-mineralization were 79.7% and 76.6%, respectively, being significantly greater than at 1 week (pSignificance: The experimental TCS-based cement, TCS 50, proved to be capable of re-mineralizing artificially demineralized dentin. The incorporation of biomimetic analogs promoted re- mineralization upon 6-week SBF storage. However, re-mineralization appeared incomplete, this even for TCS 50 to which biomimetic analogs were added and upon 6-week SBF storage.

Original languageEnglish
Pages (from-to)505-513
Number of pages9
JournalDental Materials
Volume33
Issue number5
DOIs
Publication statusPublished - May 1 2017

Fingerprint

Silicate Cement
Biomimetics
Dentin
Silicates
Cements
Body fluids
Body Fluids
Electron probe microanalysis
carbopol 940
Pemetrexed
tricalcium silicate
Third Molar
Firearms
Immersion
Zirconia
Field emission
Baths
Sodium

Keywords

  • Biomimetic analogs
  • EPMA
  • Re-mineralization
  • Tricalcium silicate

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Re-mineralizing dentin using an experimental tricalcium silicate cement with biomimetic analogs. / Li, Xin; De Munck, Jan; Yoshihara, Kumiko; Pedano, Mariano; Van Landuyt, Kirsten; Chen, Zhi; Van Meerbeek, Bart.

In: Dental Materials, Vol. 33, No. 5, 01.05.2017, p. 505-513.

Research output: Contribution to journalArticle

Li, X, De Munck, J, Yoshihara, K, Pedano, M, Van Landuyt, K, Chen, Z & Van Meerbeek, B 2017, 'Re-mineralizing dentin using an experimental tricalcium silicate cement with biomimetic analogs', Dental Materials, vol. 33, no. 5, pp. 505-513. https://doi.org/10.1016/j.dental.2017.02.003
Li, Xin ; De Munck, Jan ; Yoshihara, Kumiko ; Pedano, Mariano ; Van Landuyt, Kirsten ; Chen, Zhi ; Van Meerbeek, Bart. / Re-mineralizing dentin using an experimental tricalcium silicate cement with biomimetic analogs. In: Dental Materials. 2017 ; Vol. 33, No. 5. pp. 505-513.
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abstract = "Objectives To characterize the re-mineralization potential of an experimental zirconium oxide (ZrO2) containing tricalcium silicate (TCS) cement, TCS 50, with the incorporation of biomimetic analogs at demineralized dentin. Methods Class-I cavities were prepared in non- carious human third molars. The dentin cavities were demineralized using a pH-cycling protocol, involving 50 cyclic immersions in pH-4.8 and pH-7 baths for 0.5 h and 2.5 h, successively. The cavities were filled with TCS 50 with/without biomimetic analogs (3{\%} polyacrylic acid, 8{\%} sodium trimetaphosphate) being added to the mixed TCS 50 cement prior to application. The commercial hCSCs Biodentine (Septodont) and ProRoot MTA (Dentsply Sirona) served as controls. After 1 and 6 weeks storage in simulated body fluid (SBF), the polished specimen cross-sections were chemically characterized using a field-emission-gun Electron Probe Micro-Analysis (Feg- EPMA). Results EPMA line-scans and elemental mappings confirmed early re-mineralization induced by TCS 50 at 1 week. When biomimetic analogs were added to TCS 50, re-mineralization was more efficient after 6 weeks; the relative depth and intensity of re-mineralization were 79.7{\%} and 76.6{\%}, respectively, being significantly greater than at 1 week (pSignificance: The experimental TCS-based cement, TCS 50, proved to be capable of re-mineralizing artificially demineralized dentin. The incorporation of biomimetic analogs promoted re- mineralization upon 6-week SBF storage. However, re-mineralization appeared incomplete, this even for TCS 50 to which biomimetic analogs were added and upon 6-week SBF storage.",
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AU - Li, Xin

AU - De Munck, Jan

AU - Yoshihara, Kumiko

AU - Pedano, Mariano

AU - Van Landuyt, Kirsten

AU - Chen, Zhi

AU - Van Meerbeek, Bart

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AB - Objectives To characterize the re-mineralization potential of an experimental zirconium oxide (ZrO2) containing tricalcium silicate (TCS) cement, TCS 50, with the incorporation of biomimetic analogs at demineralized dentin. Methods Class-I cavities were prepared in non- carious human third molars. The dentin cavities were demineralized using a pH-cycling protocol, involving 50 cyclic immersions in pH-4.8 and pH-7 baths for 0.5 h and 2.5 h, successively. The cavities were filled with TCS 50 with/without biomimetic analogs (3% polyacrylic acid, 8% sodium trimetaphosphate) being added to the mixed TCS 50 cement prior to application. The commercial hCSCs Biodentine (Septodont) and ProRoot MTA (Dentsply Sirona) served as controls. After 1 and 6 weeks storage in simulated body fluid (SBF), the polished specimen cross-sections were chemically characterized using a field-emission-gun Electron Probe Micro-Analysis (Feg- EPMA). Results EPMA line-scans and elemental mappings confirmed early re-mineralization induced by TCS 50 at 1 week. When biomimetic analogs were added to TCS 50, re-mineralization was more efficient after 6 weeks; the relative depth and intensity of re-mineralization were 79.7% and 76.6%, respectively, being significantly greater than at 1 week (pSignificance: The experimental TCS-based cement, TCS 50, proved to be capable of re-mineralizing artificially demineralized dentin. The incorporation of biomimetic analogs promoted re- mineralization upon 6-week SBF storage. However, re-mineralization appeared incomplete, this even for TCS 50 to which biomimetic analogs were added and upon 6-week SBF storage.

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KW - Tricalcium silicate

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