Re-expression of NR2B-containing NMDA receptors in vitro by suppression of neuronal activity

Kazuyuki Kiyosue, Takeshi Y. Hiyama, Kimiko Nakayama, Michiki Kasai, Takahisa Taguchi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

N-methyl-D-aspartate receptors (NMDARs) are known to play critical roles in the development of the nervous system, and their expression is regulated in an activity-dependent fashion during development. However, the regulation of NMDAR expression after circuit formation is less well understood. To examine this, we performed patch-clamp recordings from chick cerebral neurons in an activity-controlled culture. Analysis of NMDAR channels from neurons before synapse formation showed that there are two components in channel open kinetics. The major slow component is clearly blocked by ifenprodil, a specific inhibitor of NR2B-containing NMDARs. In contrast, slow component of NMDAR channel opening from neurons after synapse formation became minor and ifenprodil had little effect on the NMDAR channel openings. Furthermore, this change is reversibly regulated by neuronal activity, in that suppression induces the re-expression of NR2B-containing NMDARs, even after circuit formation.

Original languageEnglish
Pages (from-to)59-65
Number of pages7
JournalInternational Journal of Developmental Neuroscience
Volume22
Issue number2
DOIs
Publication statusPublished - Apr 2004
Externally publishedYes

Fingerprint

N-Methyl-D-Aspartate Receptors
Neurons
Synapses
Nervous System
NR2B NMDA receptor
In Vitro Techniques
ifenprodil

Keywords

  • Activity-dependent regulation
  • Development
  • NR2B
  • Re-expression

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Cite this

Re-expression of NR2B-containing NMDA receptors in vitro by suppression of neuronal activity. / Kiyosue, Kazuyuki; Hiyama, Takeshi Y.; Nakayama, Kimiko; Kasai, Michiki; Taguchi, Takahisa.

In: International Journal of Developmental Neuroscience, Vol. 22, No. 2, 04.2004, p. 59-65.

Research output: Contribution to journalArticle

Kiyosue, Kazuyuki ; Hiyama, Takeshi Y. ; Nakayama, Kimiko ; Kasai, Michiki ; Taguchi, Takahisa. / Re-expression of NR2B-containing NMDA receptors in vitro by suppression of neuronal activity. In: International Journal of Developmental Neuroscience. 2004 ; Vol. 22, No. 2. pp. 59-65.
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