Rat Parathyroid Gland, with Special Reference to Its Blood Vascular Bed, Pericapillary Space and Intercellular Space

Toshihisa Tanaka, Mari Tsubouchi, Yutaka Tsubouchi, Aiji Ohtsuka, Takuro Murakami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.

Original languageEnglish
Pages (from-to)243-253
Number of pages11
JournalActa Medica Okayama
Volume50
Issue number5
Publication statusPublished - Oct 1996

Fingerprint

Parathyroid Glands
Extracellular Space
Blood Vessels
Rats
Blood
Tissue
Collagen
Basement Membrane
Colloids
Electron microscopy
Optical microscopy
Vascular Endothelium
Iron
Transmission Electron Microscopy
Electron Scanning Microscopy
Scanning electron microscopy
Enzymes
Light

Keywords

  • Cationic and anionic iron colloid stainings
  • Freeze fracture
  • Maceration
  • Parathyroid gland
  • Vascular casting

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rat Parathyroid Gland, with Special Reference to Its Blood Vascular Bed, Pericapillary Space and Intercellular Space. / Tanaka, Toshihisa; Tsubouchi, Mari; Tsubouchi, Yutaka; Ohtsuka, Aiji; Murakami, Takuro.

In: Acta Medica Okayama, Vol. 50, No. 5, 10.1996, p. 243-253.

Research output: Contribution to journalArticle

Tanaka, Toshihisa ; Tsubouchi, Mari ; Tsubouchi, Yutaka ; Ohtsuka, Aiji ; Murakami, Takuro. / Rat Parathyroid Gland, with Special Reference to Its Blood Vascular Bed, Pericapillary Space and Intercellular Space. In: Acta Medica Okayama. 1996 ; Vol. 50, No. 5. pp. 243-253.
@article{6a4715befe5a41cd929387c8402af025,
title = "Rat Parathyroid Gland, with Special Reference to Its Blood Vascular Bed, Pericapillary Space and Intercellular Space",
abstract = "The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.",
keywords = "Cationic and anionic iron colloid stainings, Freeze fracture, Maceration, Parathyroid gland, Vascular casting",
author = "Toshihisa Tanaka and Mari Tsubouchi and Yutaka Tsubouchi and Aiji Ohtsuka and Takuro Murakami",
year = "1996",
month = "10",
language = "English",
volume = "50",
pages = "243--253",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "5",

}

TY - JOUR

T1 - Rat Parathyroid Gland, with Special Reference to Its Blood Vascular Bed, Pericapillary Space and Intercellular Space

AU - Tanaka, Toshihisa

AU - Tsubouchi, Mari

AU - Tsubouchi, Yutaka

AU - Ohtsuka, Aiji

AU - Murakami, Takuro

PY - 1996/10

Y1 - 1996/10

N2 - The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.

AB - The blood vascular bed, perivascular space and intercellular space of the rat parathyroid gland were studied using scanning electron microscopy of vascular casts, freeze-cracked tissue samples, and NaOH-digested tissue blocks. The findings were supplemented by transmission light and electron microscopy of iron colloid-treated or enzyme-digested tissue sections. The rat parathyroid gland contained a rich network of capillaries. These capillaries were surrounded by marked pericapillary spaces which were demarcated by basal lamina of both capillaries and parenchymal cells. The pericapillary spaces contained numerous collagen fibrils, and issued many crista-like projections which ran deep into the sheets of parenchymal cells. The intercellular spaces of parenchymal cells contained neither basal lamina nor collagen fibrils. The surfaces of the parenchymal cells showed strong negative charging, and maintained the intercellular spaces. The luminal surfaces of the capillary endothelium also showed strong negative charging, and maintained the capillary lumen.

KW - Cationic and anionic iron colloid stainings

KW - Freeze fracture

KW - Maceration

KW - Parathyroid gland

KW - Vascular casting

UR - http://www.scopus.com/inward/record.url?scp=0030254745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030254745&partnerID=8YFLogxK

M3 - Article

C2 - 8914677

AN - SCOPUS:0030254745

VL - 50

SP - 243

EP - 253

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 5

ER -