Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage

She Hoon Oh, Kazuchika Miyoshi, Hiroaki Funahashi

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

A suitable chemically defined culture medium for 1-cell rat embryos (mR1ECM) was modified to obtain sperm penetration, and the developmental competence of oocytes fertilized in the medium was compared to that of oocytes fertilized in a traditional medium, modified Krebs-Ringer bicarbonate medium (mKRB). Sperm penetration was not observed when polyvinyl alcohol was replaced with BSA in mR1ECM (mR1ECM-BSA); the incidence was improved only when the osmolarity in mR1ECM-BSA was increased to that in mKRB (310 mOsm) by addition of NaCl. The proportion of oocytes penetrated in mR1ECM-BSA with NaCl increased (71.6 ± 6.9%), which was not different compared to that in mKRB (76.7 ± 13.7%). High incidences of sperm penetration (88.8 ± 4.1% to 93.1 ± 5.1%) were also observed when NaCl concentration in mR1ECM-BSA was increased from 76,7 mM to 100-130 mM. The incidence of embryos developing to the morula and blastocyst stages was higher when fertilized in mR1ECM-BSA containing 110-130 mM NaCl (91-94%) than in mKRB (70%). A total of 5 offspring were obtained after transfer of the morulae and blastocysts (69 embryos/7 females). These results demonstrate that a high developmental ability of rat embryos to the blastocyst stage is attained when the embryos have been fertilized in mR1ECM-BSA containing 110-130 mM NaCl and then cultured in mR1ECM.

Original languageEnglish
Pages (from-to)884-889
Number of pages6
JournalBiology of Reproduction
Volume59
Issue number4
DOIs
Publication statusPublished - Oct 1998

Fingerprint

Aptitude
Blastocyst
Bovine Serum Albumin
Sodium Chloride
Oocytes
Culture Media
Bicarbonates
Sperm-Ovum Interactions
Embryonic Structures
Cell Culture Techniques
Morula
Incidence
Polyvinyl Alcohol
Embryo Transfer
Osmolar Concentration
Mental Competency

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

@article{0832f989e9bd4899901e6906c6825228,
title = "Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage",
abstract = "A suitable chemically defined culture medium for 1-cell rat embryos (mR1ECM) was modified to obtain sperm penetration, and the developmental competence of oocytes fertilized in the medium was compared to that of oocytes fertilized in a traditional medium, modified Krebs-Ringer bicarbonate medium (mKRB). Sperm penetration was not observed when polyvinyl alcohol was replaced with BSA in mR1ECM (mR1ECM-BSA); the incidence was improved only when the osmolarity in mR1ECM-BSA was increased to that in mKRB (310 mOsm) by addition of NaCl. The proportion of oocytes penetrated in mR1ECM-BSA with NaCl increased (71.6 ± 6.9{\%}), which was not different compared to that in mKRB (76.7 ± 13.7{\%}). High incidences of sperm penetration (88.8 ± 4.1{\%} to 93.1 ± 5.1{\%}) were also observed when NaCl concentration in mR1ECM-BSA was increased from 76,7 mM to 100-130 mM. The incidence of embryos developing to the morula and blastocyst stages was higher when fertilized in mR1ECM-BSA containing 110-130 mM NaCl (91-94{\%}) than in mKRB (70{\%}). A total of 5 offspring were obtained after transfer of the morulae and blastocysts (69 embryos/7 females). These results demonstrate that a high developmental ability of rat embryos to the blastocyst stage is attained when the embryos have been fertilized in mR1ECM-BSA containing 110-130 mM NaCl and then cultured in mR1ECM.",
author = "Oh, {She Hoon} and Kazuchika Miyoshi and Hiroaki Funahashi",
year = "1998",
month = "10",
doi = "10.1095/biolreprod59.4.884",
language = "English",
volume = "59",
pages = "884--889",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Rat oocytes fertilized in modified rat 1-cell embryo culture medium containing a high sodium chloride concentration and bovine serum albumin maintain developmental ability to the blastocyst stage

AU - Oh, She Hoon

AU - Miyoshi, Kazuchika

AU - Funahashi, Hiroaki

PY - 1998/10

Y1 - 1998/10

N2 - A suitable chemically defined culture medium for 1-cell rat embryos (mR1ECM) was modified to obtain sperm penetration, and the developmental competence of oocytes fertilized in the medium was compared to that of oocytes fertilized in a traditional medium, modified Krebs-Ringer bicarbonate medium (mKRB). Sperm penetration was not observed when polyvinyl alcohol was replaced with BSA in mR1ECM (mR1ECM-BSA); the incidence was improved only when the osmolarity in mR1ECM-BSA was increased to that in mKRB (310 mOsm) by addition of NaCl. The proportion of oocytes penetrated in mR1ECM-BSA with NaCl increased (71.6 ± 6.9%), which was not different compared to that in mKRB (76.7 ± 13.7%). High incidences of sperm penetration (88.8 ± 4.1% to 93.1 ± 5.1%) were also observed when NaCl concentration in mR1ECM-BSA was increased from 76,7 mM to 100-130 mM. The incidence of embryos developing to the morula and blastocyst stages was higher when fertilized in mR1ECM-BSA containing 110-130 mM NaCl (91-94%) than in mKRB (70%). A total of 5 offspring were obtained after transfer of the morulae and blastocysts (69 embryos/7 females). These results demonstrate that a high developmental ability of rat embryos to the blastocyst stage is attained when the embryos have been fertilized in mR1ECM-BSA containing 110-130 mM NaCl and then cultured in mR1ECM.

AB - A suitable chemically defined culture medium for 1-cell rat embryos (mR1ECM) was modified to obtain sperm penetration, and the developmental competence of oocytes fertilized in the medium was compared to that of oocytes fertilized in a traditional medium, modified Krebs-Ringer bicarbonate medium (mKRB). Sperm penetration was not observed when polyvinyl alcohol was replaced with BSA in mR1ECM (mR1ECM-BSA); the incidence was improved only when the osmolarity in mR1ECM-BSA was increased to that in mKRB (310 mOsm) by addition of NaCl. The proportion of oocytes penetrated in mR1ECM-BSA with NaCl increased (71.6 ± 6.9%), which was not different compared to that in mKRB (76.7 ± 13.7%). High incidences of sperm penetration (88.8 ± 4.1% to 93.1 ± 5.1%) were also observed when NaCl concentration in mR1ECM-BSA was increased from 76,7 mM to 100-130 mM. The incidence of embryos developing to the morula and blastocyst stages was higher when fertilized in mR1ECM-BSA containing 110-130 mM NaCl (91-94%) than in mKRB (70%). A total of 5 offspring were obtained after transfer of the morulae and blastocysts (69 embryos/7 females). These results demonstrate that a high developmental ability of rat embryos to the blastocyst stage is attained when the embryos have been fertilized in mR1ECM-BSA containing 110-130 mM NaCl and then cultured in mR1ECM.

UR - http://www.scopus.com/inward/record.url?scp=0031705620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031705620&partnerID=8YFLogxK

U2 - 10.1095/biolreprod59.4.884

DO - 10.1095/biolreprod59.4.884

M3 - Article

C2 - 9746739

AN - SCOPUS:0031705620

VL - 59

SP - 884

EP - 889

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -