Rapid Isolation of Endogenous Inhibitor of Factor B

Takeshi Moriyama, Yasuko Kawamoto, Tatsuhiko Kobayashi, Takashi Inoue, Keinosuke Okamoto, Akio Mivama

Research output: Contribution to journalArticle


An inhibitor of mouse factor B was purified from lysate of L cells by a human factor B-affinity column. The purified inhibitor was found to be homogeneous with a molecular weight of about 25,000 and identical to the 25K protein isolated from L cell-liysate by the coprecipitation method with anti mouse factor B. Hemolytic titration of the activity and PAGE-analysis of the inhibitor-factor B complex showed that 1 mol of inhibitor reacted with 1 mol of mouse factor B and inactivated its hemolytic activity.

Original languageEnglish
Pages (from-to)349-357
Number of pages9
Issue number3
Publication statusPublished - Jan 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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  • Cite this

    Moriyama, T., Kawamoto, Y., Kobayashi, T., Inoue, T., Okamoto, K., & Mivama, A. (1984). Rapid Isolation of Endogenous Inhibitor of Factor B. MICROBIOLOGY and IMMUNOLOGY, 28(3), 349-357. https://doi.org/10.1111/j.1348-0421.1984.tb00686.x