Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatment of live animals with NH4Cl

Mitsuko Hayashi, Yasuhiro Nose, Taro Uyama, Yoshinori Moriyama, Hitoshi Michibata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

When the vanadium-rich ascidian Ascidia sydneiensis samea was immersed in seawater that contained 10 mM or 20 mM NH4Cl for 20 hr, the number of blood cells increased dramatically. The specific type of blood cells whose level increased was identified as the signet ring cell, the so- called vanadocyte. Identification was made not only from morphological characteristics but also by immunological staining with a monoclonal antibody, S4D5, that is specific to signet ring cells. The initial number of signet ring cells was 2.30 x 106 cells/ml of blood. The size of the subpopulation of signet ring cells increased about threefold during immersion in seawater plus 10 mM and 20 mM NH4Cl, respectively, for 20 hr. No remarkable increases were observed in the populations of the other types of blood cells. However, concentration of vanadium in the blood rose slightly during the treatment with 10 mM or 20 mM NH4Cl.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Experimental Zoology
Volume275
Issue number1
DOIs
Publication statusPublished - May 1 1996
Externally publishedYes

Fingerprint

Samea
vanadium
Ascidiacea
blood cells
animals
seawater
cells
blood cell counts
monoclonal antibodies
Rosa
blood

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatment of live animals with NH4Cl. / Hayashi, Mitsuko; Nose, Yasuhiro; Uyama, Taro; Moriyama, Yoshinori; Michibata, Hitoshi.

In: Journal of Experimental Zoology, Vol. 275, No. 1, 01.05.1996, p. 1-7.

Research output: Contribution to journalArticle

Hayashi, Mitsuko ; Nose, Yasuhiro ; Uyama, Taro ; Moriyama, Yoshinori ; Michibata, Hitoshi. / Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatment of live animals with NH4Cl. In: Journal of Experimental Zoology. 1996 ; Vol. 275, No. 1. pp. 1-7.
@article{ab029eda08474a948e76feb6b10c3727,
title = "Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatment of live animals with NH4Cl",
abstract = "When the vanadium-rich ascidian Ascidia sydneiensis samea was immersed in seawater that contained 10 mM or 20 mM NH4Cl for 20 hr, the number of blood cells increased dramatically. The specific type of blood cells whose level increased was identified as the signet ring cell, the so- called vanadocyte. Identification was made not only from morphological characteristics but also by immunological staining with a monoclonal antibody, S4D5, that is specific to signet ring cells. The initial number of signet ring cells was 2.30 x 106 cells/ml of blood. The size of the subpopulation of signet ring cells increased about threefold during immersion in seawater plus 10 mM and 20 mM NH4Cl, respectively, for 20 hr. No remarkable increases were observed in the populations of the other types of blood cells. However, concentration of vanadium in the blood rose slightly during the treatment with 10 mM or 20 mM NH4Cl.",
author = "Mitsuko Hayashi and Yasuhiro Nose and Taro Uyama and Yoshinori Moriyama and Hitoshi Michibata",
year = "1996",
month = "5",
day = "1",
doi = "10.1002/(SICI)1097-010X(19960501)275:1<1::AID-JEZ1>3.3.CO;2-2",
language = "English",
volume = "275",
pages = "1--7",
journal = "Journal of Experimental Zoology Part A: Comparative Experimental Biology",
issn = "0022-104X",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatment of live animals with NH4Cl

AU - Hayashi, Mitsuko

AU - Nose, Yasuhiro

AU - Uyama, Taro

AU - Moriyama, Yoshinori

AU - Michibata, Hitoshi

PY - 1996/5/1

Y1 - 1996/5/1

N2 - When the vanadium-rich ascidian Ascidia sydneiensis samea was immersed in seawater that contained 10 mM or 20 mM NH4Cl for 20 hr, the number of blood cells increased dramatically. The specific type of blood cells whose level increased was identified as the signet ring cell, the so- called vanadocyte. Identification was made not only from morphological characteristics but also by immunological staining with a monoclonal antibody, S4D5, that is specific to signet ring cells. The initial number of signet ring cells was 2.30 x 106 cells/ml of blood. The size of the subpopulation of signet ring cells increased about threefold during immersion in seawater plus 10 mM and 20 mM NH4Cl, respectively, for 20 hr. No remarkable increases were observed in the populations of the other types of blood cells. However, concentration of vanadium in the blood rose slightly during the treatment with 10 mM or 20 mM NH4Cl.

AB - When the vanadium-rich ascidian Ascidia sydneiensis samea was immersed in seawater that contained 10 mM or 20 mM NH4Cl for 20 hr, the number of blood cells increased dramatically. The specific type of blood cells whose level increased was identified as the signet ring cell, the so- called vanadocyte. Identification was made not only from morphological characteristics but also by immunological staining with a monoclonal antibody, S4D5, that is specific to signet ring cells. The initial number of signet ring cells was 2.30 x 106 cells/ml of blood. The size of the subpopulation of signet ring cells increased about threefold during immersion in seawater plus 10 mM and 20 mM NH4Cl, respectively, for 20 hr. No remarkable increases were observed in the populations of the other types of blood cells. However, concentration of vanadium in the blood rose slightly during the treatment with 10 mM or 20 mM NH4Cl.

UR - http://www.scopus.com/inward/record.url?scp=0029760668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029760668&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1097-010X(19960501)275:1<1::AID-JEZ1>3.3.CO;2-2

DO - 10.1002/(SICI)1097-010X(19960501)275:1<1::AID-JEZ1>3.3.CO;2-2

M3 - Article

AN - SCOPUS:0029760668

VL - 275

SP - 1

EP - 7

JO - Journal of Experimental Zoology Part A: Comparative Experimental Biology

JF - Journal of Experimental Zoology Part A: Comparative Experimental Biology

SN - 0022-104X

IS - 1

ER -