Rapid hematopoietic progenitor mobilization by sulfated colominic acid

Shiro Kubonishi, Tomoko Kikuchi, Shinya Yamaguchi, Hirokazu Tamamura, Nobutaka Fujii, Takeshi Watanabe, Fernando Arenzana-Seisdedos, Kazuma Ikeda, Toshimitsu Matsui, Mitsune Tanimoto, Yoshio Katayama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hematopoietic progenitor cells (HPCs) can be mobilized from bone marrow (BM) to the blood by G-CSF. In this process, CXCR4 and CD26 play critical roles. Sulfated colominic acid (SCA) inhibits HIV entry, the step which requires CXCR4 and CD26 as co-receptors. Thus, we hypothesized that SCA would modulate HPC trafficking. We first found that SCA mobilized HPCs rapidly via CD26-independent mechanism. In vitro progenitor migration toward chemokine SDF-1 was significantly enhanced by SCA, and it was completely abrogated by CXCR4 inhibition. This likely originated from the inhibition of CXCR4 down-regulation after interaction with SDF-1. Serum SDF-1 level increased after SCA injection, whereas no change was observed in BM and bone. These results suggest that SCA induces HPC mobilization by modulating CXCR4 function resulting in attraction toward increased SDF-1 in the circulation. Furthermore, we confirmed an additive effect with G-CSF in mobilization. SCA may provide an efficacy in clinical mobilization.

Original languageEnglish
Pages (from-to)970-975
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume355
Issue number4
DOIs
Publication statusPublished - Apr 20 2007

Keywords

  • CXCR4
  • G-CSF
  • Hematopoietic stem/progenitor cells
  • Mobilization
  • SDF-1/CXCL12
  • Sulfated colominic acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kubonishi, S., Kikuchi, T., Yamaguchi, S., Tamamura, H., Fujii, N., Watanabe, T., Arenzana-Seisdedos, F., Ikeda, K., Matsui, T., Tanimoto, M., & Katayama, Y. (2007). Rapid hematopoietic progenitor mobilization by sulfated colominic acid. Biochemical and Biophysical Research Communications, 355(4), 970-975. https://doi.org/10.1016/j.bbrc.2007.02.069