TY - JOUR
T1 - Rapid hematopoietic progenitor mobilization by sulfated colominic acid
AU - Kubonishi, Shiro
AU - Kikuchi, Tomoko
AU - Yamaguchi, Shinya
AU - Tamamura, Hirokazu
AU - Fujii, Nobutaka
AU - Watanabe, Takeshi
AU - Arenzana-Seisdedos, Fernando
AU - Ikeda, Kazuma
AU - Matsui, Toshimitsu
AU - Tanimoto, Mitsune
AU - Katayama, Yoshio
N1 - Funding Information:
This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology (Grant No. 17790644 to Y.K.).
PY - 2007/4/20
Y1 - 2007/4/20
N2 - Hematopoietic progenitor cells (HPCs) can be mobilized from bone marrow (BM) to the blood by G-CSF. In this process, CXCR4 and CD26 play critical roles. Sulfated colominic acid (SCA) inhibits HIV entry, the step which requires CXCR4 and CD26 as co-receptors. Thus, we hypothesized that SCA would modulate HPC trafficking. We first found that SCA mobilized HPCs rapidly via CD26-independent mechanism. In vitro progenitor migration toward chemokine SDF-1 was significantly enhanced by SCA, and it was completely abrogated by CXCR4 inhibition. This likely originated from the inhibition of CXCR4 down-regulation after interaction with SDF-1. Serum SDF-1 level increased after SCA injection, whereas no change was observed in BM and bone. These results suggest that SCA induces HPC mobilization by modulating CXCR4 function resulting in attraction toward increased SDF-1 in the circulation. Furthermore, we confirmed an additive effect with G-CSF in mobilization. SCA may provide an efficacy in clinical mobilization.
AB - Hematopoietic progenitor cells (HPCs) can be mobilized from bone marrow (BM) to the blood by G-CSF. In this process, CXCR4 and CD26 play critical roles. Sulfated colominic acid (SCA) inhibits HIV entry, the step which requires CXCR4 and CD26 as co-receptors. Thus, we hypothesized that SCA would modulate HPC trafficking. We first found that SCA mobilized HPCs rapidly via CD26-independent mechanism. In vitro progenitor migration toward chemokine SDF-1 was significantly enhanced by SCA, and it was completely abrogated by CXCR4 inhibition. This likely originated from the inhibition of CXCR4 down-regulation after interaction with SDF-1. Serum SDF-1 level increased after SCA injection, whereas no change was observed in BM and bone. These results suggest that SCA induces HPC mobilization by modulating CXCR4 function resulting in attraction toward increased SDF-1 in the circulation. Furthermore, we confirmed an additive effect with G-CSF in mobilization. SCA may provide an efficacy in clinical mobilization.
KW - CXCR4
KW - G-CSF
KW - Hematopoietic stem/progenitor cells
KW - Mobilization
KW - SDF-1/CXCL12
KW - Sulfated colominic acid
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U2 - 10.1016/j.bbrc.2007.02.069
DO - 10.1016/j.bbrc.2007.02.069
M3 - Article
C2 - 17335773
AN - SCOPUS:33847647968
SN - 0006-291X
VL - 355
SP - 970
EP - 975
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
