Pruebas diagnósticas rápidas frente a la RT-PCR para detectar infecciones por el virus del Ébola: revisión sistemática y metanálisis

Translated title of the contribution: Rapid diagnostic tests versus RT–PCR for Ebola virus infections: a systematic review and meta-analysis

Basilua Andre Muzembo, Kei Kitahara, Ayumu Ohno, Ngangu Patrick Ntontolo, Nlandu Roger Ngatu, Keinosuke Okamoto, Shin Ichi Miyoshi

Research output: Contribution to journalReview articlepeer-review

Abstract

Objective To evaluate the clinical accuracy of rapid diagnostic tests for the detection of Ebola virus. Methods We searched MEDLINE®, Embase® and Web of Science for articles published between 1976 and October 2021 reporting on clinical studies assessing the performance of Ebola virus rapid diagnostic tests compared with reverse transcription polymerase chain reaction (RT–PCR). We assessed study quality using the QUADAS-2 criteria. To estimate the pooled sensitivity and specificity of these rapid diagnostic tests, we used a bivariate random-effects meta-analysis. Findings Our search identified 113 unique studies, of which nine met the inclusion criteria. The studies were conducted in the Democratic Republic of the Congo, Guinea, Liberia and Sierra Leone and they evaluated 12 rapid diagnostic tests. We included eight studies in the meta-analysis. The pooled sensitivity and specificity of the rapid tests were 86% (95% confidence interval, CI: 80–91) and 95% (95% CI: 91–97), respectively. However, pooled sensitivity decreased to 83% (95% CI: 77–88) after removing outliers. Pooled sensitivity increased to 90% (95% CI: 82–94) when analysis was restricted to studies using the RT–PCR from altona Diagnostics as gold standard. Pooled sensitivity increased to 99% (95% CI: 67–100) when the analysis was restricted to studies using whole or capillary blood specimens. Conclusion The included rapid diagnostic tests did not detect all the Ebola virus disease cases. While the sensitivity and specificity of these tests are moderate, they are still valuable tools, especially useful for triage and detecting Ebola virus in remote areas.

Translated title of the contributionRapid diagnostic tests versus RT–PCR for Ebola virus infections: a systematic review and meta-analysis
Original languageSpanish
Pages (from-to)447-458
Number of pages12
JournalBulletin of the World Health Organization
Volume100
Issue number7
DOIs
Publication statusPublished - Jul 1 2022

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

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