Rapid determination of multidrug resistance-associated protein in cancer cells by capillary electrophoresis immunoassay

Julius Mbuna, Takashi Kaneta, Totaro Imasaka

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The adenosine triphosphate (ATP) binding-cassette (ABC) transporters are a superfamily of cellular proteins that have been partly implicated as a cause of multidrug resistance (MDR) in cancer cells. The ABC superfamily consists of P-glycoprotein, multidrug resistance-associated proteins (MRP) and breast cancer-related proteins, of which MRP is of particular interest because of its ability to efflux a broader range of substrates. Since MRP1 is the most prominent member of the MRP family, a simple technique is needed for its quantification. We developed a simple, fast (total analysis time of 3h) capillary electrophoresis immunoassay (CEIA) for the quantification of MRP1 in cancer cells. MRP1 antibody was labeled with fluorescein isothiocyanate. The labeled antibody was incubated with the cell lysate for a fixed interval (1h), after which the cell lysate mixture was directly injected into the capillary to separate the complex of MRP1 and its antibody from free antibody. The noncompetitive CEIA method had a limit of detection of 0.2nM and a good linear range (1.7-14.9×104 cells), and was fairly reproducible (RSD

Original languageEnglish
Pages (from-to)3923-3927
Number of pages5
JournalJournal of Chromatography A
Volume1218
Issue number25
DOIs
Publication statusPublished - Jun 24 2011

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Multidrug Resistance-Associated Proteins
Capillary electrophoresis
Capillary Electrophoresis
Immunoassay
Cells
Antibodies
Neoplasms
Adenosine Triphosphate
P-Glycoprotein
Fluorescein
Multiple Drug Resistance
Proteins
Limit of Detection
Breast Neoplasms
Substrates

Keywords

  • Cancer cell
  • Capillary electrophoresis immunoassay
  • Doxorubicin
  • Laser-induced fluorescence
  • Multidrug resistance-associated protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Organic Chemistry
  • Biochemistry

Cite this

Rapid determination of multidrug resistance-associated protein in cancer cells by capillary electrophoresis immunoassay. / Mbuna, Julius; Kaneta, Takashi; Imasaka, Totaro.

In: Journal of Chromatography A, Vol. 1218, No. 25, 24.06.2011, p. 3923-3927.

Research output: Contribution to journalArticle

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