Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding

Vered Tzin, Yuko Hojo, Susan R. Strickler, Lee J. Bartsch, Cairo M. Archer, Kevin R. Ahern, Shaoqun Zhou, Shawn A. Christensen, Ivan Galis, Lukas A. Mueller, Georg Jander

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Insects such as the beet armyworm (Spodoptera exigua) cause extensive damage to maize (Zea mays). Maize plants respond by triggering defense signaling, changes in gene expression, and biosynthesis of specialized metabolites. Leaves of maize inbred line B73, which has an available genome sequence, were infested with S. exigua for 1 to 24 h, followed by comparisons of the transcript and metabolite profiles with those of uninfested controls. The most extensive gene expression responses occurred rapidly, within 4-6 h after caterpillar infestation. However, both gene expression and metabolite profiles were altered within 1 h and continued to change during the entire 24 h experiment. The defensive functions of three caterpillar-induced genes were examined using available Dissociation transposon insertions in maize inbred line W22. Whereas mutations in the benzoxazinoid biosynthesis pathway (Bx1 and Bx2) significantly improved caterpillar growth, the knockout of a 13-lipoxygenase (Lox8) involved in jasmonic acid biosynthesis did not. Interestingly, 9-lipoxygenases, which lead to the production of maize death acids, were more strongly induced by caterpillar feeding than 13-lipoxygenases, suggesting an as yet unknown function in maize defense against herbivory. Together, these results provide a comprehensive view of the dynamic transcriptomic and metabolomic responses of maize leaves to caterpillar feeding.

Original languageEnglish
Pages (from-to)4709-4723
Number of pages15
JournalJournal of Experimental Botany
Volume68
Issue number16
DOIs
Publication statusPublished - Jul 20 2017

Fingerprint

Spodoptera
Spodoptera exigua
Zea mays
insect larvae
corn
lipoxygenase
leaves
biosynthesis
metabolites
gene expression
inbred lines
benzoxazinoids
Benzoxazines
Lipoxygenases
Gene Expression
metabolomics
Herbivory
Beta vulgaris
Metabolomics
jasmonic acid

Keywords

  • Benzoxazinoid
  • insect herbivore
  • jasmonic acid
  • metabolite profile
  • RNAseq
  • Spodoptera exigua
  • time course
  • transcriptome
  • Zea mays

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Tzin, V., Hojo, Y., Strickler, S. R., Bartsch, L. J., Archer, C. M., Ahern, K. R., ... Jander, G. (2017). Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding. Journal of Experimental Botany, 68(16), 4709-4723. https://doi.org/10.1093/jxb/erx274

Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding. / Tzin, Vered; Hojo, Yuko; Strickler, Susan R.; Bartsch, Lee J.; Archer, Cairo M.; Ahern, Kevin R.; Zhou, Shaoqun; Christensen, Shawn A.; Galis, Ivan; Mueller, Lukas A.; Jander, Georg.

In: Journal of Experimental Botany, Vol. 68, No. 16, 20.07.2017, p. 4709-4723.

Research output: Contribution to journalArticle

Tzin, V, Hojo, Y, Strickler, SR, Bartsch, LJ, Archer, CM, Ahern, KR, Zhou, S, Christensen, SA, Galis, I, Mueller, LA & Jander, G 2017, 'Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding', Journal of Experimental Botany, vol. 68, no. 16, pp. 4709-4723. https://doi.org/10.1093/jxb/erx274
Tzin V, Hojo Y, Strickler SR, Bartsch LJ, Archer CM, Ahern KR et al. Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding. Journal of Experimental Botany. 2017 Jul 20;68(16):4709-4723. https://doi.org/10.1093/jxb/erx274
Tzin, Vered ; Hojo, Yuko ; Strickler, Susan R. ; Bartsch, Lee J. ; Archer, Cairo M. ; Ahern, Kevin R. ; Zhou, Shaoqun ; Christensen, Shawn A. ; Galis, Ivan ; Mueller, Lukas A. ; Jander, Georg. / Rapid defense responses in maize leaves induced by Spodoptera exigua caterpillar feeding. In: Journal of Experimental Botany. 2017 ; Vol. 68, No. 16. pp. 4709-4723.
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