Rapid cytoplasmic alkalization and dynamics of intracellular compartmentation of inorganic phosphate during adaptation against salt stress in a halotolerant unicellular green alga dunaliella tertiolecta: 31P-nuclear magnetic resonance study

Kazuyuki Kuchitsu, Maki Katsuhara, Shigetoh Miyachi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.

Original languageEnglish
Pages (from-to)407-414
Number of pages8
JournalPlant and Cell Physiology
Volume30
Issue number3
Publication statusPublished - Apr 1989
Externally publishedYes

Fingerprint

Salt Stress
Green Algae
Dunaliella tertiolecta
alkalinization
phosphate (inorganic)
Chlorophyta
Nuclear Magnetic Resonance
cytoplasm
Algae
Phosphate
green alga
salt stress
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
Phosphates
Magnetic Resonance Spectroscopy
Salts
Nuclear magnetic resonance
phosphate group
phosphates

Keywords

  • 31P-nuclear magnetic resonance (NMR)
  • Dunaliella tertiolecta (Chlorophyta)
  • Inorganic phosphate (Pj)
  • Intracellular pH (cytoplasm, vacuole)
  • Osmoregulation
  • Salt stress

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

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abstract = "By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.",
keywords = "31P-nuclear magnetic resonance (NMR), Dunaliella tertiolecta (Chlorophyta), Inorganic phosphate (Pj), Intracellular pH (cytoplasm, vacuole), Osmoregulation, Salt stress",
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T2 - 31P-nuclear magnetic resonance study

AU - Kuchitsu, Kazuyuki

AU - Katsuhara, Maki

AU - Miyachi, Shigetoh

PY - 1989/4

Y1 - 1989/4

N2 - By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.

AB - By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.

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KW - Salt stress

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