Abstract
By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.
| Original language | English |
|---|---|
| Pages (from-to) | 407-414 |
| Number of pages | 8 |
| Journal | Plant and Cell Physiology |
| Volume | 30 |
| Issue number | 3 |
| Publication status | Published - Apr 1989 |
| Externally published | Yes |
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Keywords
- 31P-nuclear magnetic resonance (NMR)
- Dunaliella tertiolecta (Chlorophyta)
- Inorganic phosphate (Pj)
- Intracellular pH (cytoplasm, vacuole)
- Osmoregulation
- Salt stress
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Rapid cytoplasmic alkalization and dynamics of intracellular compartmentation of inorganic phosphate during adaptation against salt stress in a halotolerant unicellular green alga dunaliella tertiolecta : 31P-nuclear magnetic resonance study. / Kuchitsu, Kazuyuki; Katsuhara, Maki; Miyachi, Shigetoh.
In: Plant and Cell Physiology, Vol. 30, No. 3, 04.1989, p. 407-414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid cytoplasmic alkalization and dynamics of intracellular compartmentation of inorganic phosphate during adaptation against salt stress in a halotolerant unicellular green alga dunaliella tertiolecta
T2 - 31P-nuclear magnetic resonance study
AU - Kuchitsu, Kazuyuki
AU - Katsuhara, Maki
AU - Miyachi, Shigetoh
PY - 1989/4
Y1 - 1989/4
N2 - By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.
AB - By 31P-in vivo nuclear magnetic resonance spectroscopy, two intracellular compartments were detected, and their pH values were estimated in intact cells of a halotolerant unicellular green alga Dunaliella tertiolecta. They were identified as the cytoplasm (pH 7.1) and vacuoles (pH 6.0). Vacuoles were also visualized with a fluorescence-differential interference microscope. During the adaptation to the salt stress (NaCl concentration from 0.17 to 1.0 M) where cells rapidly synthesize glycerol as osmoticum, both cytoplasmic and vacuolar pH showed transient increases to about 8 and 6.5, respectively. Subsequently, cytoplasmic inorganic phosphate level, as well as sugar phosphates and terminal phosphate group levels increased. These drastic changes in chemical environment in the cytoplasm including pH and inorganic phosphate concentration are discussed to be key factors for osmoregulation that activate the synthesis and inhibit the breakdown of glycerol.
KW - 31P-nuclear magnetic resonance (NMR)
KW - Dunaliella tertiolecta (Chlorophyta)
KW - Inorganic phosphate (Pj)
KW - Intracellular pH (cytoplasm, vacuole)
KW - Osmoregulation
KW - Salt stress
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UR - http://www.scopus.com/inward/citedby.url?scp=0009124511&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0009124511
VL - 30
SP - 407
EP - 414
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 3
ER -