Rapid changes in polyphosphoinositide metabolism in pea in response to fungal signals

Kazuhiro Toyoda, Tomonori Shiraishi, Tetsuji Yamada, Yuki Ichinose, Hachiro Oku

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Effects of the elicitor and/or suppressor from Mycosphaerella pinodes on polyphosphoinositide metabolism (PI metabolism) in pea were examined both in vivo and in vitro. The elicitor induced a rapid and biphasic increase in levels of phosphatidylinositol-4,5-bisphosphate (PtdInsP2) and inositol 1,4,5-trisphosphate (IP3) in epicotyl tissues that was apparent within 15 min. A transient increase in levels of PtdInsP2 and IP3 was detected immediately in elicitor-treated plasma membranes. However, the concomitant presence of suppressor with elicitor resulted in inhibition of these increases both in vivo and in vitro. These findings suggest that the elicitor rapidly activates phosphatidylinositol kinase, phosphatidylinositol-4-monophosphate kinase and phospholipase C, which are involved in PI metabolism, whereas the suppressor markedly inhibits these enzymes. Neomycin, a known inhibitor of phospholipase C, blocked the elicitor-induced accumulation both of IP3 and pisatin and it also induced local susceptibility in pea tissues that resembled that of the fungal suppressor. From these results, it appears that rapid changes in PI metabolism are indispensable in the signal transduction related to defense responses of pea plants.

Original languageEnglish
Pages (from-to)729-735
Number of pages7
JournalPlant and Cell Physiology
Volume34
Issue number5
Publication statusPublished - Jul 1993

Fingerprint

Phosphatidylinositol Phosphates
Peas
Phosphatidylinositol 4,5-Diphosphate
Metabolism
peas
metabolism
Type C Phospholipases
Phosphatidylinositols
Tissue
Signal transduction
Inositol 1,4,5-Trisphosphate
Plasma Membrane
1-Phosphatidylinositol 4-Kinase
Neomycin
Signal Transduction
phospholipase C
phosphatidylinositols
Cell membranes
Susceptibility
Inhibitor

Keywords

  • Elicitor
  • Neomycin
  • Pea (Pisum sativum L.)
  • Phospholipase C
  • Polyphosphoinositide metabolism (PI metabolism)
  • Suppressor

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Rapid changes in polyphosphoinositide metabolism in pea in response to fungal signals. / Toyoda, Kazuhiro; Shiraishi, Tomonori; Yamada, Tetsuji; Ichinose, Yuki; Oku, Hachiro.

In: Plant and Cell Physiology, Vol. 34, No. 5, 07.1993, p. 729-735.

Research output: Contribution to journalArticle

Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Yamada, Tetsuji ; Ichinose, Yuki ; Oku, Hachiro. / Rapid changes in polyphosphoinositide metabolism in pea in response to fungal signals. In: Plant and Cell Physiology. 1993 ; Vol. 34, No. 5. pp. 729-735.
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