Rapid and simultaneous measurement of estrone, estradiol, estriol and estetrol in serum by high performance liquid chromatography with electrochemical detection

N. Hayashi, Kei Hayata, K. Sekiba

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9 Citations (Scopus)

Abstract

A high performance liquid chromatographic (HPLC) method with electrochemical detection (ECD) was developed for the simultaneous measurement of estrone, estradiol, estriol and estetrol in serum. These hormones were extracted with diethylether, chromatographed on an silica-octadecyl silane (ODS) column with an eluent of phosphate buffer solution-acetonitrile-methanol (volume ratio 152 : 85 : 40), and detected by ECD at + 1.0 V vs. Ag/AgCl. In comparisons between the values measured by this method and radioimmunoassay, significant correlations were noted for estrone (r = 0.759, p < 0.01), estradiol (r = 0.816, p < 0.001) and estriol (r = 0.830, p < 0.001). In clinical applications of this method, differences between cases of the normal and the anencephalic pregnancy in the thirty-eighth week of gestation were distinct not only in the individual estrogen, but also in the profile analysis of estrogens. With this method, all 4 serum estrogens above approximately 500 pg/ml could be measured within 2h, and the method seemed to be clinically applicable.

Original languageEnglish
Pages (from-to)143-153
Number of pages11
JournalActa Medica Okayama
Volume39
Issue number2
Publication statusPublished - 1985

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Estetrol
Estriol
Estrone
High performance liquid chromatography
Estradiol
Estrogens
High Pressure Liquid Chromatography
Serum
Silanes
Silicon Dioxide
Methanol
Buffers
Pregnancy
Phosphates
Hormones
Liquids
Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

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abstract = "A high performance liquid chromatographic (HPLC) method with electrochemical detection (ECD) was developed for the simultaneous measurement of estrone, estradiol, estriol and estetrol in serum. These hormones were extracted with diethylether, chromatographed on an silica-octadecyl silane (ODS) column with an eluent of phosphate buffer solution-acetonitrile-methanol (volume ratio 152 : 85 : 40), and detected by ECD at + 1.0 V vs. Ag/AgCl. In comparisons between the values measured by this method and radioimmunoassay, significant correlations were noted for estrone (r = 0.759, p < 0.01), estradiol (r = 0.816, p < 0.001) and estriol (r = 0.830, p < 0.001). In clinical applications of this method, differences between cases of the normal and the anencephalic pregnancy in the thirty-eighth week of gestation were distinct not only in the individual estrogen, but also in the profile analysis of estrogens. With this method, all 4 serum estrogens above approximately 500 pg/ml could be measured within 2h, and the method seemed to be clinically applicable.",
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AU - Hayata, Kei

AU - Sekiba, K.

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N2 - A high performance liquid chromatographic (HPLC) method with electrochemical detection (ECD) was developed for the simultaneous measurement of estrone, estradiol, estriol and estetrol in serum. These hormones were extracted with diethylether, chromatographed on an silica-octadecyl silane (ODS) column with an eluent of phosphate buffer solution-acetonitrile-methanol (volume ratio 152 : 85 : 40), and detected by ECD at + 1.0 V vs. Ag/AgCl. In comparisons between the values measured by this method and radioimmunoassay, significant correlations were noted for estrone (r = 0.759, p < 0.01), estradiol (r = 0.816, p < 0.001) and estriol (r = 0.830, p < 0.001). In clinical applications of this method, differences between cases of the normal and the anencephalic pregnancy in the thirty-eighth week of gestation were distinct not only in the individual estrogen, but also in the profile analysis of estrogens. With this method, all 4 serum estrogens above approximately 500 pg/ml could be measured within 2h, and the method seemed to be clinically applicable.

AB - A high performance liquid chromatographic (HPLC) method with electrochemical detection (ECD) was developed for the simultaneous measurement of estrone, estradiol, estriol and estetrol in serum. These hormones were extracted with diethylether, chromatographed on an silica-octadecyl silane (ODS) column with an eluent of phosphate buffer solution-acetonitrile-methanol (volume ratio 152 : 85 : 40), and detected by ECD at + 1.0 V vs. Ag/AgCl. In comparisons between the values measured by this method and radioimmunoassay, significant correlations were noted for estrone (r = 0.759, p < 0.01), estradiol (r = 0.816, p < 0.001) and estriol (r = 0.830, p < 0.001). In clinical applications of this method, differences between cases of the normal and the anencephalic pregnancy in the thirty-eighth week of gestation were distinct not only in the individual estrogen, but also in the profile analysis of estrogens. With this method, all 4 serum estrogens above approximately 500 pg/ml could be measured within 2h, and the method seemed to be clinically applicable.

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