Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR

Shoji Nagao, Mitsuo Yoshinouchi, Yasunari Miyagi, Atsushi Hongo, Junichi Kodama, Sachio Itou, Takafumi Kudo

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.

Original languageEnglish
Pages (from-to)863-867
Number of pages5
JournalJournal of Clinical Microbiology
Volume40
Issue number3
DOIs
Publication statusPublished - 2002

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Human papillomavirus 16
Real-Time Polymerase Chain Reaction
DNA
Cervical Intraepithelial Neoplasia
Genes
Disease Progression
Theoretical Models

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR. / Nagao, Shoji; Yoshinouchi, Mitsuo; Miyagi, Yasunari; Hongo, Atsushi; Kodama, Junichi; Itou, Sachio; Kudo, Takafumi.

In: Journal of Clinical Microbiology, Vol. 40, No. 3, 2002, p. 863-867.

Research output: Contribution to journalArticle

Nagao, Shoji ; Yoshinouchi, Mitsuo ; Miyagi, Yasunari ; Hongo, Atsushi ; Kodama, Junichi ; Itou, Sachio ; Kudo, Takafumi. / Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 3. pp. 863-867.
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