Abstract
A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.
| Original language | English |
|---|---|
| Pages (from-to) | 863-867 |
| Number of pages | 5 |
| Journal | Journal of Clinical Microbiology |
| Volume | 40 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2002 |
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ASJC Scopus subject areas
- Microbiology (medical)
- Microbiology
Cite this
Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR. / Nagao, Shoji; Yoshinouchi, Mitsuo; Miyagi, Yasunari; Hongo, Atsushi; Kodama, Junichi; Itou, Sachio; Kudo, Takafumi.
In: Journal of Clinical Microbiology, Vol. 40, No. 3, 2002, p. 863-867.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR
AU - Nagao, Shoji
AU - Yoshinouchi, Mitsuo
AU - Miyagi, Yasunari
AU - Hongo, Atsushi
AU - Kodama, Junichi
AU - Itou, Sachio
AU - Kudo, Takafumi
PY - 2002
Y1 - 2002
N2 - A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.
AB - A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.
UR - http://www.scopus.com/inward/record.url?scp=0036192470&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036192470&partnerID=8YFLogxK
U2 - 10.1128/JCM.40.3.863-867.2002
DO - 10.1128/JCM.40.3.863-867.2002
M3 - Article
C2 - 11880406
AN - SCOPUS:0036192470
VL - 40
SP - 863
EP - 867
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 3
ER -