Rapid and sensitive detection of physical status of human papillomavirus type 16 DNA by quantitative real-time PCR

Shoji Nagao, Mitsuo Yoshinouchi, Yasunari Miyagi, Atsushi Hongo, Junichi Kodama, Sachio Itoh, Takafumi Kudo

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A rapid quantitative real-time PCR method was employed to quantify the copy number of E2 and E6 genes for analysis of the physical status of human papillomavirus type 16 (HPV-16) DNA. Significant differences with respect to both copy numbers were found when more than 40% of HPV-16 DNA was integrated with disruption of the E2 gene in an experimental model. The physical status of HPV-16 DNA in 50 clinical samples was exclusively episomal in 21 cases (42%), concomitant in 14 cases (28%), and integrated in 15 cases (30%). The prevalence of integrated and/or concomitant forms of HPV-16 DNA increased with progression of cervical disease. Four of 11 cervical intraepithelial neoplasia involved integrated forms of HPV-16 DNA partially or exclusively. This rapid, sensitive technique is useful in the analysis of the physical status of HPV DNA.

Original languageEnglish
Pages (from-to)863-867
Number of pages5
JournalJournal of Clinical Microbiology
Issue number3
Publication statusPublished - Mar 20 2002


ASJC Scopus subject areas

  • Microbiology (medical)

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