Rapid and semiquantitative analysis of HSP72 and HSC73 heat shock mRNAs by mimic RT-PCR

Koji Abe, K. Kogure, Y. Itoyama

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The amounts of mRNAs for 72 kDa (HSP72) and 73 kDa (HSC73) heat shock proteins were measured semiquantitatively with competitive polymerase chain reaction (PCR) techniques after reverse transcription (RT) using a heterologous DNA fragment (PCR mimic) as an internal standard. The changes of signal intensities of PCR products were well correlated with the amount of mRNA in rat brain measured as optical densities of Northern blot. Thus, an analysis with mimic RT-PCR techique provides a rapid, semiquantitative, and safe method to detect changes of HSP72 and HSC73 mRNAs after brain ischemia.

Original languageEnglish
Pages (from-to)251-253
Number of pages3
JournalBrain Research
Volume683
Issue number2
DOIs
Publication statusPublished - Jun 19 1995
Externally publishedYes

Fingerprint

Reverse Transcription
Shock
Hot Temperature
Polymerase Chain Reaction
Messenger RNA
DNA-Directed DNA Polymerase
Heat-Shock Proteins
Brain Ischemia
Northern Blotting
Brain

Keywords

  • HSC73
  • HSP72
  • Ischemia
  • Mimic RT-PCR
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Rapid and semiquantitative analysis of HSP72 and HSC73 heat shock mRNAs by mimic RT-PCR. / Abe, Koji; Kogure, K.; Itoyama, Y.

In: Brain Research, Vol. 683, No. 2, 19.06.1995, p. 251-253.

Research output: Contribution to journalArticle

Abe, Koji ; Kogure, K. ; Itoyama, Y. / Rapid and semiquantitative analysis of HSP72 and HSC73 heat shock mRNAs by mimic RT-PCR. In: Brain Research. 1995 ; Vol. 683, No. 2. pp. 251-253.
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