Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization

Xian Zhang; Akio Tani; Fusako Kawai; Kazuhide Kimbara

doi:10.1016/j.jbiosc.2010.07.004

Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization

Xian Zhang, Akio Tani, Fusako Kawai, Kazuhide Kimbara

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria.

Original language	English
Pages (from-to)	716-719
Number of pages	4
Journal	Journal of Bioscience and Bioengineering
Volume	110
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2010.07.004
Publication status	Published - Dec 2010

Keywords

Anaerobic benzene degradation
Fluorescent in situ hybridization
Fluorospheres
Live/dead staining
Physiological status

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiosc.2010.07.004

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title = "Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization",

abstract = "Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria.",

keywords = "Anaerobic benzene degradation, Fluorescent in situ hybridization, Fluorospheres, Live/dead staining, Physiological status",

author = "Xian Zhang and Akio Tani and Fusako Kawai and Kazuhide Kimbara",

note = "Funding Information: We are most grateful to Professor H. Nojiri and Dr. M. Shintani of the Biotechnology Research Center, The University of Tokyo, for kindly providing P. putida strain KT2440 and the plasmid pCAR1::rfp. This work was partly supported by NEDO (New Energy and Industrial Technology Development Organization) .",

year = "2010",

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doi = "10.1016/j.jbiosc.2010.07.004",

language = "English",

volume = "110",

pages = "716--719",

journal = "Journal of Bioscience and Bioengineering",

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publisher = "The Society for Biotechnology, Japan",

number = "6",

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TY - JOUR

T1 - Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization

AU - Zhang, Xian

AU - Tani, Akio

AU - Kawai, Fusako

AU - Kimbara, Kazuhide

N1 - Funding Information: We are most grateful to Professor H. Nojiri and Dr. M. Shintani of the Biotechnology Research Center, The University of Tokyo, for kindly providing P. putida strain KT2440 and the plasmid pCAR1::rfp. This work was partly supported by NEDO (New Energy and Industrial Technology Development Organization) .

PY - 2010/12

Y1 - 2010/12

N2 - Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria.

AB - Quantitative analysis of the target microorganism in microbial communities is important for the assessment of bacterial activity in environment. Here we present a method of a combination of fluorescent in situ hybridization (FISH) method and live/dead staining which allows in situ identification and analysis of physiological status of specific bacteria.

KW - Anaerobic benzene degradation

KW - Fluorescent in situ hybridization

KW - Fluorospheres

KW - Live/dead staining

KW - Physiological status

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DO - 10.1016/j.jbiosc.2010.07.004

M3 - Article

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AN - SCOPUS:78449241261

SN - 1389-1723

VL - 110

SP - 716

EP - 719

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 6

ER -

Rapid and multiple in situ identification and analyses of physiological status of specific bacteria based on fluorescent in situ hybridization

Abstract

Keywords

ASJC Scopus subject areas

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