RAPD markers linked to the tomato mosaic virus resistance gene, Tm-1, in tomato

Taku Ohmori, Minora Murata, Fusao Motoyoshi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Random amplified polymorphic DNA (RAPD) markers were screened in two nearly isogenic lines (NILs) of tomato, one of which carried Tm-1 gene conferring resistance to tomato mosaic virus (ToMV) and the other carried its susceptible allele. Among 1030 polymerase chain reaction (PCR) products generated by using 220 different 10-base oligonucleotide primers, 12 fragments were polymorphic between the NILs. Six markers arbitrarily chosen from these 12 fragments were examined whether they link to the Tm-1 in 125 BC1 plants. No recombinations were detected between the five markers and the Tm-1. The other one marker was also proved to link to the Tm-1, but their genetic distance was not determined due to some difficulty in distinguishing the RAPD band from the adjacent PCR products.

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalJapanese Journal of Genetics
Volume70
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this