Rantes and MCP-1 expression in antigen challenged guinea-pig airways

M. L. Watson, E. M. Campbell, A. M. White, Teizo Yoshimura, J. Westwick

Research output: Contribution to journalArticle

Abstract

Allergic asthma is associated with a pronounced accumulation of eosinophils and mononuclear cells in the airways. In this study we have examined the expression of CC chemokine mRNA in the lungs of sensitised guinea-pigs following challenge with aerosolised antigen. Northern blot analysis of total lung mRNA expression indicated that MCP-1 was markedly upregulated 2, 6 and 12h after antigen challenge of sensitised, but not naive, animals, whilst RANTES mRNA expression did not exhibit marked changes in either group at these time points. Semiquantitative RT-PCR of whole lung mRNA demonstrated a 4 fold increase in MCP-1 mRNA expression (p

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - Mar 20 1998
Externally publishedYes

Fingerprint

1-methylcyclopropene
guinea pigs
Guinea Pigs
lungs
antigens
Antigens
Messenger RNA
Lung
asthma
eosinophils
Northern blotting
Chemokine CCL5
CC Chemokines
reverse transcriptase polymerase chain reaction
Eosinophils
Northern Blotting
Animals
Asthma
animals
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Watson, M. L., Campbell, E. M., White, A. M., Yoshimura, T., & Westwick, J. (1998). Rantes and MCP-1 expression in antigen challenged guinea-pig airways. FASEB Journal, 12(5).

Rantes and MCP-1 expression in antigen challenged guinea-pig airways. / Watson, M. L.; Campbell, E. M.; White, A. M.; Yoshimura, Teizo; Westwick, J.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Watson, ML, Campbell, EM, White, AM, Yoshimura, T & Westwick, J 1998, 'Rantes and MCP-1 expression in antigen challenged guinea-pig airways', FASEB Journal, vol. 12, no. 5.
Watson ML, Campbell EM, White AM, Yoshimura T, Westwick J. Rantes and MCP-1 expression in antigen challenged guinea-pig airways. FASEB Journal. 1998 Mar 20;12(5).
Watson, M. L. ; Campbell, E. M. ; White, A. M. ; Yoshimura, Teizo ; Westwick, J. / Rantes and MCP-1 expression in antigen challenged guinea-pig airways. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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