Abstract
The spread of penicillin non-susceptible Streptococcus pneumoniae (PNSSP) is an emerging problem for the treatment of acute otitis media (AOM). Attendance of children at day care centers, as well as the spread of PNSSP, is a risk factor for AOM. The status of the spread of PNSSP during the acute infection phase of AOM has not been evaluated. We examined the clonality of samples from seven children in a day care center who simultaneously developed AOM caused by PNSSP. The seven isolates from the children, and six control samples were grouped by serotyping, by determining resistance to antimicrobial agents, and by genotyping, carried out by sequencer-based random amplified polymorphic DNA (RAPD), and validated by bootstrap analysis. There was no evidence to indicate the direct dissemination of PNSSP among these patients in the day care center, although the simultaneous occurrence of PNSSP AOM had initially suggested a clonal outbreak. The possible presence of a common ancestral strain suggested the importance of surveillance during the carrier state. The result of RAPD genotyping was highly reproducible, as validated by the high bootstrap score. The use of an automated sequencer, in combination with a careful choice of primers, and commercially established kits, played a significant role in the reproducibility of the studies.
| Original language | English |
|---|---|
| Pages (from-to) | 239-242 |
| Number of pages | 4 |
| Journal | Journal of Infection and Chemotherapy |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2001 |
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Keywords
- Acute otitis media
- Day care center
- Penicillin non-susceptible Streptococcus pneumoniae (PNSSP)
- Random amplified polymorphic DNA (RAPD)
ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Random amplified polymorphic DNA analysis applied to acute otitis media caused by penicillin non-susceptible Streptococcus pneumoniae. / Nakashima, T.; Fukushima, K.; Tahara, Makoto; Sugata, K.; Ogawa, T.; Sugata, A.; Gunduz, M.; Ueki, Y.; Uno, Y.; Nishizaki, Kazunori.
In: Journal of Infection and Chemotherapy, Vol. 7, No. 4, 2001, p. 239-242.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Random amplified polymorphic DNA analysis applied to acute otitis media caused by penicillin non-susceptible Streptococcus pneumoniae
AU - Nakashima, T.
AU - Fukushima, K.
AU - Tahara, Makoto
AU - Sugata, K.
AU - Ogawa, T.
AU - Sugata, A.
AU - Gunduz, M.
AU - Ueki, Y.
AU - Uno, Y.
AU - Nishizaki, Kazunori
PY - 2001
Y1 - 2001
N2 - The spread of penicillin non-susceptible Streptococcus pneumoniae (PNSSP) is an emerging problem for the treatment of acute otitis media (AOM). Attendance of children at day care centers, as well as the spread of PNSSP, is a risk factor for AOM. The status of the spread of PNSSP during the acute infection phase of AOM has not been evaluated. We examined the clonality of samples from seven children in a day care center who simultaneously developed AOM caused by PNSSP. The seven isolates from the children, and six control samples were grouped by serotyping, by determining resistance to antimicrobial agents, and by genotyping, carried out by sequencer-based random amplified polymorphic DNA (RAPD), and validated by bootstrap analysis. There was no evidence to indicate the direct dissemination of PNSSP among these patients in the day care center, although the simultaneous occurrence of PNSSP AOM had initially suggested a clonal outbreak. The possible presence of a common ancestral strain suggested the importance of surveillance during the carrier state. The result of RAPD genotyping was highly reproducible, as validated by the high bootstrap score. The use of an automated sequencer, in combination with a careful choice of primers, and commercially established kits, played a significant role in the reproducibility of the studies.
AB - The spread of penicillin non-susceptible Streptococcus pneumoniae (PNSSP) is an emerging problem for the treatment of acute otitis media (AOM). Attendance of children at day care centers, as well as the spread of PNSSP, is a risk factor for AOM. The status of the spread of PNSSP during the acute infection phase of AOM has not been evaluated. We examined the clonality of samples from seven children in a day care center who simultaneously developed AOM caused by PNSSP. The seven isolates from the children, and six control samples were grouped by serotyping, by determining resistance to antimicrobial agents, and by genotyping, carried out by sequencer-based random amplified polymorphic DNA (RAPD), and validated by bootstrap analysis. There was no evidence to indicate the direct dissemination of PNSSP among these patients in the day care center, although the simultaneous occurrence of PNSSP AOM had initially suggested a clonal outbreak. The possible presence of a common ancestral strain suggested the importance of surveillance during the carrier state. The result of RAPD genotyping was highly reproducible, as validated by the high bootstrap score. The use of an automated sequencer, in combination with a careful choice of primers, and commercially established kits, played a significant role in the reproducibility of the studies.
KW - Acute otitis media
KW - Day care center
KW - Penicillin non-susceptible Streptococcus pneumoniae (PNSSP)
KW - Random amplified polymorphic DNA (RAPD)
UR - http://www.scopus.com/inward/record.url?scp=18244392013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18244392013&partnerID=8YFLogxK
U2 - 10.1007/s101560170019
DO - 10.1007/s101560170019
M3 - Article
C2 - 11810590
AN - SCOPUS:18244392013
VL - 7
SP - 239
EP - 242
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
SN - 1341-321X
IS - 4
ER -