Ralstonia solanacearum type III secretion system effector Rip36 induces a hypersensitive response in the nonhost wild eggplant Solanum torvum

Kamrun Nahar, Iyo Matsumoto, Fumiko Taguchi, Yoshishige Inagaki, Mikihiro Yamamoto, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Takafumi Mukaihara

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Abstract

Summary: Ralstonia solanacearum is a Gram-negative soil-borne bacterium that causes bacterial wilt disease in more than 200 plant species, including economically important Solanaceae species. In R.solanacearum, the hypersensitive response and pathogenicity (Hrp) type III secretion system is required for both the ability to induce the hypersensitive response (HR) in nonhost plants and pathogenicity in host plants. Recently, 72 effector genes, called rip (Ralstonia protein injected into plant cells), have been identified in R.solanacearumRS1000. RS1002, a spontaneous nalixidic acid-resistant derivative of RS1000, induced strong HR in the nonhost wild eggplant Solanum torvum in an Hrp-dependent manner. An Agrobacterium-mediated transient expression system revealed that Rip36, a putative Zn-dependent protease effector of R.solanacearum, induced HR in S.torvum. A mutation in the putative Zn-binding motif (E149A) completely abolished the ability to induce HR. In agreement with this result, the RS1002-derived Δrip36 and rip36E149A mutants lost the ability to induce HR in S.torvum. An E149A mutation had no effect on the translocation of Rip36 into plant cells. These results indicate that Rip36 is an avirulent factor that induces HR in S.torvum and that a putative Zn-dependent protease motif is essential for this activity.

Original languageEnglish
Pages (from-to)297-303
Number of pages7
JournalMolecular Plant Pathology
Volume15
Issue number3
DOIs
Publication statusPublished - Apr 1 2014

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ASJC Scopus subject areas

  • Molecular Biology
  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

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