Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells

Masataka Oita, Yoshihiro Uto, Masahide Tominaga, Motoharu Sasaki, Yasuo Hara, Taro Kishi, Hitoshi Hori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background/Aim: The aims of this study were to evaluate the cell survival uncertainty distribution of radiation and to assess the accuracy of predictions of tumor response by using three different in vitro experimental cell cultures with radiosensitizers (including etanidazole). Materials and Methods: Using EMT6 cells and X-rays, the cell survival fraction was obtained from 15, 34, and 21 different experiments under normoxic, hypoxic, and hypoxic-plusradiosensitizer culture, respectively. Results: The αcoefficients were 0.257±0.188, 0.078±0.080, and 0.182±0.116 Gy-1, respectively. The β coefficients were 0.0159±0.0208, 0.0076±0.0113, and 0.0062±0.0077 Gy-2, respectively. The αcoefficient and the dose that killed half of the clonogens population (D50) were significantly different between normoxic cell and hypoxic cell cultures (p

Original languageEnglish
Pages (from-to)4621-4626
Number of pages6
JournalAnticancer Research
Volume34
Issue number8
Publication statusPublished - Aug 1 2014

Fingerprint

Radiation Tolerance
Uncertainty
Etanidazole
Cell Survival
Cell Culture Techniques
X-Rays
Radiation
Population
Neoplasms
In Vitro Techniques

Keywords

  • Biophysical modelling
  • In vitro
  • Radiosensitivity uncertainty
  • Radiotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Oita, M., Uto, Y., Tominaga, M., Sasaki, M., Hara, Y., Kishi, T., & Hori, H. (2014). Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells. Anticancer Research, 34(8), 4621-4626.

Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells. / Oita, Masataka; Uto, Yoshihiro; Tominaga, Masahide; Sasaki, Motoharu; Hara, Yasuo; Kishi, Taro; Hori, Hitoshi.

In: Anticancer Research, Vol. 34, No. 8, 01.08.2014, p. 4621-4626.

Research output: Contribution to journalArticle

Oita, M, Uto, Y, Tominaga, M, Sasaki, M, Hara, Y, Kishi, T & Hori, H 2014, 'Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells', Anticancer Research, vol. 34, no. 8, pp. 4621-4626.
Oita M, Uto Y, Tominaga M, Sasaki M, Hara Y, Kishi T et al. Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells. Anticancer Research. 2014 Aug 1;34(8):4621-4626.
Oita, Masataka ; Uto, Yoshihiro ; Tominaga, Masahide ; Sasaki, Motoharu ; Hara, Yasuo ; Kishi, Taro ; Hori, Hitoshi. / Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells. In: Anticancer Research. 2014 ; Vol. 34, No. 8. pp. 4621-4626.
@article{3ba0ef48f13448689c184dcede1b6952,
title = "Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells",
abstract = "Background/Aim: The aims of this study were to evaluate the cell survival uncertainty distribution of radiation and to assess the accuracy of predictions of tumor response by using three different in vitro experimental cell cultures with radiosensitizers (including etanidazole). Materials and Methods: Using EMT6 cells and X-rays, the cell survival fraction was obtained from 15, 34, and 21 different experiments under normoxic, hypoxic, and hypoxic-plusradiosensitizer culture, respectively. Results: The αcoefficients were 0.257±0.188, 0.078±0.080, and 0.182±0.116 Gy-1, respectively. The β coefficients were 0.0159±0.0208, 0.0076±0.0113, and 0.0062±0.0077 Gy-2, respectively. The αcoefficient and the dose that killed half of the clonogens population (D50) were significantly different between normoxic cell and hypoxic cell cultures (p",
keywords = "Biophysical modelling, In vitro, Radiosensitivity uncertainty, Radiotherapy",
author = "Masataka Oita and Yoshihiro Uto and Masahide Tominaga and Motoharu Sasaki and Yasuo Hara and Taro Kishi and Hitoshi Hori",
year = "2014",
month = "8",
day = "1",
language = "English",
volume = "34",
pages = "4621--4626",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "8",

}

TY - JOUR

T1 - Radiosensitivity uncertainty evaluation for the in vitro biophysical modeling of EMT6 cells

AU - Oita, Masataka

AU - Uto, Yoshihiro

AU - Tominaga, Masahide

AU - Sasaki, Motoharu

AU - Hara, Yasuo

AU - Kishi, Taro

AU - Hori, Hitoshi

PY - 2014/8/1

Y1 - 2014/8/1

N2 - Background/Aim: The aims of this study were to evaluate the cell survival uncertainty distribution of radiation and to assess the accuracy of predictions of tumor response by using three different in vitro experimental cell cultures with radiosensitizers (including etanidazole). Materials and Methods: Using EMT6 cells and X-rays, the cell survival fraction was obtained from 15, 34, and 21 different experiments under normoxic, hypoxic, and hypoxic-plusradiosensitizer culture, respectively. Results: The αcoefficients were 0.257±0.188, 0.078±0.080, and 0.182±0.116 Gy-1, respectively. The β coefficients were 0.0159±0.0208, 0.0076±0.0113, and 0.0062±0.0077 Gy-2, respectively. The αcoefficient and the dose that killed half of the clonogens population (D50) were significantly different between normoxic cell and hypoxic cell cultures (p

AB - Background/Aim: The aims of this study were to evaluate the cell survival uncertainty distribution of radiation and to assess the accuracy of predictions of tumor response by using three different in vitro experimental cell cultures with radiosensitizers (including etanidazole). Materials and Methods: Using EMT6 cells and X-rays, the cell survival fraction was obtained from 15, 34, and 21 different experiments under normoxic, hypoxic, and hypoxic-plusradiosensitizer culture, respectively. Results: The αcoefficients were 0.257±0.188, 0.078±0.080, and 0.182±0.116 Gy-1, respectively. The β coefficients were 0.0159±0.0208, 0.0076±0.0113, and 0.0062±0.0077 Gy-2, respectively. The αcoefficient and the dose that killed half of the clonogens population (D50) were significantly different between normoxic cell and hypoxic cell cultures (p

KW - Biophysical modelling

KW - In vitro

KW - Radiosensitivity uncertainty

KW - Radiotherapy

UR - http://www.scopus.com/inward/record.url?scp=84908669475&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908669475&partnerID=8YFLogxK

M3 - Article

C2 - 25075109

AN - SCOPUS:84908669475

VL - 34

SP - 4621

EP - 4626

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 8

ER -