TY - JOUR
T1 - RAD-seq-Based High-Density Linkage Map Construction and QTL Mapping of Biomass-Related Traits in Sorghum using the Japanese Landrace Takakibi NOG
AU - Kajiya-Kanegae, Hiromi
AU - Takanashi, Hideki
AU - Fujimoto, Masaru
AU - Ishimori, Motoyuki
AU - Ohnishi, Norikazu
AU - Fiona, Wacera W.
AU - Omollo, Everlyne A.
AU - Kobayashi, Masaaki
AU - Yano, Kentaro
AU - Nakano, Michiharu
AU - Kozuka, Toshiaki
AU - Kusaba, Makoto
AU - Iwata, Hiroyoshi
AU - Tsutsumi, Nobuhiro
AU - Sakamoto, Wataru
N1 - Funding Information:
This work was supported by Core Research for Evolutional Science and Technology (CREST) from Japan Science and Technology Agency (JST) (to N.T. and to W.S.); the JST-Mirai Program (Grant No. JPMJMI18E to N.T. and W.S.); KAKENHI grants from the Ministry of Education, Culture, Sports, Science and Technology (16H06554 to W.S.); KAKENHI grants from the Japan Society for the Promotion of Science (JSPS) (17H01457 to N.T. and 18K19343 to W.S.); and Oohara Foundation (to W.S.).
Publisher Copyright:
©The Author(s) 2020. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Sorghum [Sorghum bicolor (L.) Moench] grown locally by Japanese farmers is generically termed Takakibi, although its genetic diversity compared with geographically distant varieties or even within Takakibi lines remains unclear. To explore the genomic diversity and genetic traits controlling biomass and other physiological traits in Takakibi, we focused on a landrace, NOG, in this study. Admixture analysis of 460 sorghum accessions revealed that NOG belonged to the subgroup that represented Asian sorghums, and it was only distantly related to American/African accessions including BTx623. In an attempt to dissect major traits related to biomass, we generated a recombinant inbred line (RIL) froma cross between BTx623 and NOG, and we constructed a highdensity linkage map based on 3,710 single-nucleotide polymorphisms obtained by restriction-site-associated DNA sequencing of 213 RIL individuals. Consequently, 13 fine quantitative trait loci (QTLs) were detected on chromosomes 2, 3, 6, 7, 8 and 9, which included five QTLs for days to heading, three for plant height (PH) and total shoot fresh weight and two for Brix. Furthermore, we identified two dominant loci for PH as being identical to the previously reported dw1 and dw3. Together, these results corroborate the diversified genome of Japanese Takakibi, while the RIL population and high-density linkage map generated in this study will be useful for dissecting other important traits in sorghum.
AB - Sorghum [Sorghum bicolor (L.) Moench] grown locally by Japanese farmers is generically termed Takakibi, although its genetic diversity compared with geographically distant varieties or even within Takakibi lines remains unclear. To explore the genomic diversity and genetic traits controlling biomass and other physiological traits in Takakibi, we focused on a landrace, NOG, in this study. Admixture analysis of 460 sorghum accessions revealed that NOG belonged to the subgroup that represented Asian sorghums, and it was only distantly related to American/African accessions including BTx623. In an attempt to dissect major traits related to biomass, we generated a recombinant inbred line (RIL) froma cross between BTx623 and NOG, and we constructed a highdensity linkage map based on 3,710 single-nucleotide polymorphisms obtained by restriction-site-associated DNA sequencing of 213 RIL individuals. Consequently, 13 fine quantitative trait loci (QTLs) were detected on chromosomes 2, 3, 6, 7, 8 and 9, which included five QTLs for days to heading, three for plant height (PH) and total shoot fresh weight and two for Brix. Furthermore, we identified two dominant loci for PH as being identical to the previously reported dw1 and dw3. Together, these results corroborate the diversified genome of Japanese Takakibi, while the RIL population and high-density linkage map generated in this study will be useful for dissecting other important traits in sorghum.
KW - Biomass
KW - Quantitative trait locus
KW - Recombinant inbred line
KW - Restriction-site-associated DNA sequencing
KW - Sorghum
KW - Takakibi
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U2 - 10.1093/PCP/PCAA056
DO - 10.1093/PCP/PCAA056
M3 - Article
C2 - 32353144
AN - SCOPUS:85088609888
VL - 61
SP - 1262
EP - 1272
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -