RAD-seq-Based High-Density Linkage Map Construction and QTL Mapping of Biomass-Related Traits in Sorghum using the Japanese Landrace Takakibi NOG

Hiromi Kajiya-Kanegae, Hideki Takanashi, Masaru Fujimoto, Motoyuki Ishimori, Norikazu Ohnishi, Fiona Wacera W, Everlyne A. Omollo, Masaaki Kobayashi, Kentaro Yano, Michiharu Nakano, Toshiaki Kozuka, Makoto Kusaba, Hiroyoshi Iwata, Nobuhiro Tsutsumi, Wataru Sakamoto

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Sorghum [Sorghum bicolor (L.) Moench] grown locally by Japanese farmers is generically termed Takakibi, although its genetic diversity compared with geographically distant varieties or even within Takakibi lines remains unclear. To explore the genomic diversity and genetic traits controlling biomass and other physiological traits in Takakibi, we focused on a landrace, NOG, in this study. Admixture analysis of 460 sorghum accessions revealed that NOG belonged to the subgroup that represented Asian sorghums, and it was only distantly related to American/African accessions including BTx623. In an attempt to dissect major traits related to biomass, we generated a recombinant inbred line (RIL) from a cross between BTx623 and NOG, and we constructed a high-density linkage map based on 3,710 single-nucleotide polymorphisms obtained by restriction-site-associated DNA sequencing of 213 RIL individuals. Consequently, 13 fine quantitative trait loci (QTLs) were detected on chromosomes 2, 3, 6, 7, 8 and 9, which included five QTLs for days to heading, three for plant height (PH) and total shoot fresh weight and two for Brix. Furthermore, we identified two dominant loci for PH as being identical to the previously reported dw1 and dw3. Together, these results corroborate the diversified genome of Japanese Takakibi, while the RIL population and high-density linkage map generated in this study will be useful for dissecting other important traits in sorghum.

Original languageEnglish
Pages (from-to)1262-1272
Number of pages11
JournalPlant & cell physiology
Volume61
Issue number7
DOIs
Publication statusPublished - Jul 1 2020

Keywords

  • Biomass
  • Quantitative trait locus
  • Recombinant inbred line
  • Restriction-site-associated DNA sequencing
  • Sorghum
  • Takakibi

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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