Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis

Akihiro C E Shibata, Limin H. Chen, Rie Nagai, Fumiyoshi Ishidate, Rahul Chadda, Yoshihiro Miwa, Keiji Naruse, Yuki M. Shirai, Takahiro K. Fujiwara, Akihiro Kusumi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The focal adhesion (FA) is an integrin-based structure built in/on the plasma membrane (PM), linking the extracellular matrix to the actin stress-fibers, working as cell migration scaffolds. Previously, we proposed the archipelago architecture of the FA, in which FA largely consists of fluid membrane, dotted with small islands accumulating FA proteins: membrane molecules enter the inter-island channels in the FA zone rather freely, and the integrins in the FA-protein islands rapidly exchanges with those in the bulk membrane. Here, we examined how Rac1, a small G-protein regulating FA formation, and its activators αPIX and βPIX, are recruited to the FA zones. PIX molecules are recruited from the cytoplasm to the FA zones directly. In contrast, majorities of Rac1 molecules first arrive from the cytoplasm on the general inner PM surface, and then enter the FA zones via lateral diffusion on the PM, which is possible due to rapid Rac1 diffusion even within the FA zones, slowed only by a factor of two to four compared with that outside. The constitutively-active Rac1 mutant exhibited temporary and all-time immobilizations in the FA zone, suggesting that upon PIX-induced Rac1 activation at the FA-protein islands, Rac1 tends to be immobilized at the FA-protein islands.

Original languageEnglish
Pages (from-to)161-177
Number of pages17
JournalCytoskeleton
Volume70
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Focal Adhesions
Membranes
Islands
Cell Membrane
Integrins
Channel Islands
Cytoplasm
rac1 GTP-Binding Protein
Stress Fibers
Monomeric GTP-Binding Proteins
Immobilization
Cell Movement
Extracellular Matrix
Actins

Keywords

  • Archipelago architecture
  • Diffusion
  • Percolation
  • PIX
  • Single fluorescent-molecule imaging/tracking

ASJC Scopus subject areas

  • Cell Biology
  • Structural Biology

Cite this

Shibata, A. C. E., Chen, L. H., Nagai, R., Ishidate, F., Chadda, R., Miwa, Y., ... Kusumi, A. (2013). Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis. Cytoskeleton, 70(3), 161-177. https://doi.org/10.1002/cm.21097

Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis. / Shibata, Akihiro C E; Chen, Limin H.; Nagai, Rie; Ishidate, Fumiyoshi; Chadda, Rahul; Miwa, Yoshihiro; Naruse, Keiji; Shirai, Yuki M.; Fujiwara, Takahiro K.; Kusumi, Akihiro.

In: Cytoskeleton, Vol. 70, No. 3, 03.2013, p. 161-177.

Research output: Contribution to journalArticle

Shibata, ACE, Chen, LH, Nagai, R, Ishidate, F, Chadda, R, Miwa, Y, Naruse, K, Shirai, YM, Fujiwara, TK & Kusumi, A 2013, 'Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis', Cytoskeleton, vol. 70, no. 3, pp. 161-177. https://doi.org/10.1002/cm.21097
Shibata, Akihiro C E ; Chen, Limin H. ; Nagai, Rie ; Ishidate, Fumiyoshi ; Chadda, Rahul ; Miwa, Yoshihiro ; Naruse, Keiji ; Shirai, Yuki M. ; Fujiwara, Takahiro K. ; Kusumi, Akihiro. / Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis. In: Cytoskeleton. 2013 ; Vol. 70, No. 3. pp. 161-177.
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