Quick two-step RNA ligation employing periodate oxidation.

Shinya Kurata, Takashi Ohtsuki, Tsutomu Suzuki, Kimitsuna Watanabe

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.

Original languageEnglish
JournalNucleic acids research.
Volume31
Issue number22
Publication statusPublished - Nov 15 2003
Externally publishedYes

Fingerprint

Ligation
RNA
Nucleotides
metaperiodate
Oxides

ASJC Scopus subject areas

  • Genetics

Cite this

Quick two-step RNA ligation employing periodate oxidation. / Kurata, Shinya; Ohtsuki, Takashi; Suzuki, Tsutomu; Watanabe, Kimitsuna.

In: Nucleic acids research., Vol. 31, No. 22, 15.11.2003.

Research output: Contribution to journalArticle

Kurata, S, Ohtsuki, T, Suzuki, T & Watanabe, K 2003, 'Quick two-step RNA ligation employing periodate oxidation.', Nucleic acids research., vol. 31, no. 22.
Kurata, Shinya ; Ohtsuki, Takashi ; Suzuki, Tsutomu ; Watanabe, Kimitsuna. / Quick two-step RNA ligation employing periodate oxidation. In: Nucleic acids research. 2003 ; Vol. 31, No. 22.
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