Abstract
The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.
Original language | English |
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Pages (from-to) | e145 |
Journal | Nucleic acids research |
Volume | 31 |
Issue number | 22 |
DOIs | |
Publication status | Published - Nov 15 2003 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics