Quick two-step RNA ligation employing periodate oxidation.

Shinya Kurata, Takashi Ohtsuki, Tsutomu Suzuki, Kimitsuna Watanabe

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.

Original languageEnglish
Pages (from-to)e145
JournalNucleic acids research
Volume31
Issue number22
Publication statusPublished - Nov 15 2003
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Kurata, S., Ohtsuki, T., Suzuki, T., & Watanabe, K. (2003). Quick two-step RNA ligation employing periodate oxidation. Nucleic acids research, 31(22), e145.