Abstract
The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.
| Original language | English |
|---|---|
| Journal | Nucleic acids research. |
| Volume | 31 |
| Issue number | 22 |
| Publication status | Published - Nov 15 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Quick two-step RNA ligation employing periodate oxidation. / Kurata, Shinya; Ohtsuki, Takashi; Suzuki, Tsutomu; Watanabe, Kimitsuna.
In: Nucleic acids research., Vol. 31, No. 22, 15.11.2003.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Quick two-step RNA ligation employing periodate oxidation.
AU - Kurata, Shinya
AU - Ohtsuki, Takashi
AU - Suzuki, Tsutomu
AU - Watanabe, Kimitsuna
PY - 2003/11/15
Y1 - 2003/11/15
N2 - The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.
AB - The introduction of modified or labeled nucleotides into RNA is a powerful RNA engineering tool as it enables us to investigate how native RNA modifications affect RNA function and structure. It also helps in the structural analysis of RNA. A modified nucleotide can be introduced into a specific position of RNA by the method of two-step enzymatic ligation of RNA fragments. However, this method requires a complicated purification step between the two ligation steps that results in low yields of the ligation product. Here we have developed a new ligation technique employing periodate oxide that eliminates this purification step. This increases the total yield of the ligation product and makes it a faster procedure.
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M3 - Article
VL - 31
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 22
ER -