Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number.

Shuichiro Sato, Yuji Noguchi, Hisashi Wada, Shoichiro Fujita, Shinichiro Nakamura, Ryo Tanaka, Tetsuya Nakada, Kosei Hasegawa, Kazuhiko Nakagawa, Fumihito Koizumi, Toshiro Ono, Kazuhiro Nouso, Achim Jungbluth, Yao Tseng Chen, Lloyd J. Old, Yasushi Shiratori, Eiichi Nakayama

Research output: Contribution to journalArticle

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Abstract

We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGE-1a mRNA.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalInternational Journal of Oncology
Volume26
Issue number1
Publication statusPublished - Jan 2005

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Real-Time Polymerase Chain Reaction
Messenger RNA
Neoplasms
Proteins
Liver Neoplasms
Esophageal Neoplasms
Placenta
Immunohistochemistry
Testis
Pancreas
Ovary
Prostatic Neoplasms
Staining and Labeling
Liver

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number. / Sato, Shuichiro; Noguchi, Yuji; Wada, Hisashi; Fujita, Shoichiro; Nakamura, Shinichiro; Tanaka, Ryo; Nakada, Tetsuya; Hasegawa, Kosei; Nakagawa, Kazuhiko; Koizumi, Fumihito; Ono, Toshiro; Nouso, Kazuhiro; Jungbluth, Achim; Chen, Yao Tseng; Old, Lloyd J.; Shiratori, Yasushi; Nakayama, Eiichi.

In: International Journal of Oncology, Vol. 26, No. 1, 01.2005, p. 57-63.

Research output: Contribution to journalArticle

Sato, S, Noguchi, Y, Wada, H, Fujita, S, Nakamura, S, Tanaka, R, Nakada, T, Hasegawa, K, Nakagawa, K, Koizumi, F, Ono, T, Nouso, K, Jungbluth, A, Chen, YT, Old, LJ, Shiratori, Y & Nakayama, E 2005, 'Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number.', International Journal of Oncology, vol. 26, no. 1, pp. 57-63.
Sato, Shuichiro ; Noguchi, Yuji ; Wada, Hisashi ; Fujita, Shoichiro ; Nakamura, Shinichiro ; Tanaka, Ryo ; Nakada, Tetsuya ; Hasegawa, Kosei ; Nakagawa, Kazuhiko ; Koizumi, Fumihito ; Ono, Toshiro ; Nouso, Kazuhiro ; Jungbluth, Achim ; Chen, Yao Tseng ; Old, Lloyd J. ; Shiratori, Yasushi ; Nakayama, Eiichi. / Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number. In: International Journal of Oncology. 2005 ; Vol. 26, No. 1. pp. 57-63.
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abstract = "We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGE-1a mRNA.",
author = "Shuichiro Sato and Yuji Noguchi and Hisashi Wada and Shoichiro Fujita and Shinichiro Nakamura and Ryo Tanaka and Tetsuya Nakada and Kosei Hasegawa and Kazuhiko Nakagawa and Fumihito Koizumi and Toshiro Ono and Kazuhiro Nouso and Achim Jungbluth and Chen, {Yao Tseng} and Old, {Lloyd J.} and Yasushi Shiratori and Eiichi Nakayama",
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T1 - Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number.

AU - Sato, Shuichiro

AU - Noguchi, Yuji

AU - Wada, Hisashi

AU - Fujita, Shoichiro

AU - Nakamura, Shinichiro

AU - Tanaka, Ryo

AU - Nakada, Tetsuya

AU - Hasegawa, Kosei

AU - Nakagawa, Kazuhiko

AU - Koizumi, Fumihito

AU - Ono, Toshiro

AU - Nouso, Kazuhiro

AU - Jungbluth, Achim

AU - Chen, Yao Tseng

AU - Old, Lloyd J.

AU - Shiratori, Yasushi

AU - Nakayama, Eiichi

PY - 2005/1

Y1 - 2005/1

N2 - We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGE-1a mRNA.

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