Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number.

Shuichiro Sato, Yuji Noguchi, Hisashi Wada, Shoichiro Fujita, Shinichiro Nakamura, Ryo Tanaka, Tetsuya Nakada, Kosei Hasegawa, Kazuhiko Nakagawa, Fumihito Koizumi, Toshiro Ono, Kazuhiro Nouso, Achim Jungbluth, Yao Tseng Chen, Lloyd J. Old, Yasushi Shiratori, Eiichi Nakayama

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11 Citations (Scopus)

Abstract

We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGE-1a mRNA.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalInternational journal of oncology
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 2005

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Sato, S., Noguchi, Y., Wada, H., Fujita, S., Nakamura, S., Tanaka, R., Nakada, T., Hasegawa, K., Nakagawa, K., Koizumi, F., Ono, T., Nouso, K., Jungbluth, A., Chen, Y. T., Old, L. J., Shiratori, Y., & Nakayama, E. (2005). Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number. International journal of oncology, 26(1), 57-63. https://doi.org/10.3892/ijo.26.1.57