Quantitative proteomic analysis of the response to zinc, magnesium, and calcium deficiency in specific cell types of Arabidopsis roots

Yoichiro Fukao, Mami Kobayashi, Sajad Majeed Zargar, Rie Kurata, Risa Fukui, Izumi C. Mori, Yoshiyuki Ogata

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    The proteome profiles of specific cell types have recently been investigated using techniques such as fluorescence activated cell sorting and laser capture microdissection. However, quantitative proteomic analysis of specific cell types has not yet been performed. In this study, to investigate the response of the proteome to zinc, magnesium, and calcium deficiency in specific cell types of Arabidopsis thaliana roots, we performed isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics using GFP-expressing protoplasts collected by fluorescence-activated cell sorting. Protoplasts were collected from the pGL2-GFPer and pMGP-GFPer marker lines for epidermis or inner cell lines (pericycle, endodermis, and cortex), respectively. To increase the number of proteins identified, iTRAQ-labeled peptides were separated into 24 fractions by OFFGFEL electrophoresis prior to high-performance liquid chromatography coupled with mass spectrometry analysis. Overall, 1039 and 737 proteins were identified and quantified in the epidermal and inner cell lines, respectively. Interestingly, the expression of many proteins was decreased in the epidermis by mineral deficiency, although a weaker effect was observed in inner cell lines such as the pericycle, endodermis, and cortex. Here, we report for the first time the quantitative proteomics of specific cell types in Arabidopsis roots.

    Original languageEnglish
    Article number1
    JournalProteomes
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - Mar 1 2016

    Keywords

    • 14-3-3 protein family
    • Arabidopsis thaliana
    • FACS
    • ITRAQ
    • OFFGEL electrophoresis
    • Root specific cell types

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Clinical Biochemistry

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