Quantitative nature of overexpression experiments

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Abstract

Overexpression experiments are sometimes considered as qualitative experiments designed to identify novel proteins and study their function. However, in order to draw conclusions regarding protein overexpression through association analyses using large-scale biological data sets, we need to recognize the quantitative nature of overexpression experiments. Here I discuss the quantitative features of two different types of overexpression experiment: absolute and relative. I also introduce the four primary mechanisms involved in growth defects caused by protein overexpression: resource overload, stoichiometric imbalance, promiscuous interactions, and pathway modulation associated with the degree of overexpression.

Original languageEnglish
Pages (from-to)3932-3939
Number of pages8
JournalMolecular Biology of the Cell
Volume26
Issue number22
DOIs
Publication statusPublished - Nov 5 2015

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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