Quantitative Evaluation of the Therapeutic Effects of Antibiotics Using Silkworms Infected with Human Pathogenic Microorganisms

Hiroshi Hamamoto, Kenji Kurokawa, Chikara Kaito, Koushirou Kamura, Iony Manitra Razanajatovo, Hiroyuki Kusuhara, Tomofumi Santa, Kazuhisa Sekimizu

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)

Abstract

The injection of bacteria (Staphylococcus aureus, Stenotrophomonas maltophilia) or true fungi (Candida albicans, Candida tropicalis) that are pathogenic to humans into the silkworm hemolymph leads to death of the larvae within 2 days. Antibiotics used for clinical purposes have therapeutic effects on silkworms infected with these pathogens. The 50% effective doses obtained by injection into the silkworm hemolymph are consistent with those reported for mice. Injection of vancomycin and kanamycin into the silkworm hemolymph was effective, but oral administration was not. Chloramphenicol, which is effective by oral administration, appeared in the silkworm hemolymph soon after injection into the midgut, whereas vancomycin did not. Isolated midgut membranes were impermeable to vancomycin. Thus, the ineffectiveness of oral administration of vancomycin to silkworms is due to a lack of intestinal absorption.

Original languageEnglish
Pages (from-to)774-779
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 2004

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Quantitative Evaluation of the Therapeutic Effects of Antibiotics Using Silkworms Infected with Human Pathogenic Microorganisms'. Together they form a unique fingerprint.

Cite this