Quantitative evaluation of nano-order micromotion of cultured cells using electric cell-substrate impedance sensing method

Noriko Goda, Y. Yamamoto, N. Kataoka, Takao Nakamura, Toshimasa Kusuhara, S. Mohri, Keiji Naruse, F. Kajiya

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Electric cell-substrate impedance sensing (ECIS) system can detect electrically micro-motion of the cell cultured in the gold electrode, originally developed by I. Giaever et al. The ECIS system makes data continuously on resistance and capacitance values change in electric impedance by the cell micro-motion at real time. Human umbilical vein endothelial cells were cultured on the ECIS electrode, and measured after chemical substance (estrogen) stimulation electric impedance over 40 hours. A parameter analyzed cellular motion by using the precise mathematical model simulated cell electric impedance based on the Cole-Cole model. This mathematical model agreed with the experiment value over wide frequency range 25 Hz to 60 kHz. Impedance of the mathematical model in the ECIS system varied with the change in cell-cell distance A and cell-substrate distance h in the motion of the cultured cell. The parameter for the distance of A and h was defined as SA and S h to do the simple and easy evaluation of the cell micro-motion. Furthermore, the actual dimension evaluation of A, h was tired from these parameters. A change in impedance following estrogen stimulation was analyzed by using the mathematical model, and changes in A and h were estimated. As a result, it was proved that about 120 nm decreased A, and about 2 nm increased h after 40 hours.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages122-125
Number of pages4
Volume17 IFMBE
Publication statusPublished - 2007
Event13th International Conference on Electrical Bioimpedance and the 8th Conference on Electrical Impedance Tomography 2007, ICEBI 2007 - Graz, Austria
Duration: Aug 29 2007Sep 2 2007

Other

Other13th International Conference on Electrical Bioimpedance and the 8th Conference on Electrical Impedance Tomography 2007, ICEBI 2007
CountryAustria
CityGraz
Period8/29/079/2/07

Fingerprint

Electric batteries
Cells
Mathematical models
Substrates
Estrogens
Electrodes
Endothelial cells
Gold
Capacitance
Experiments

Keywords

  • Bio-electrical impedance
  • ECIS method
  • Endothelial cells
  • Estrogen
  • Mathematical model

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Goda, N., Yamamoto, Y., Kataoka, N., Nakamura, T., Kusuhara, T., Mohri, S., ... Kajiya, F. (2007). Quantitative evaluation of nano-order micromotion of cultured cells using electric cell-substrate impedance sensing method. In IFMBE Proceedings (Vol. 17 IFMBE, pp. 122-125)

Quantitative evaluation of nano-order micromotion of cultured cells using electric cell-substrate impedance sensing method. / Goda, Noriko; Yamamoto, Y.; Kataoka, N.; Nakamura, Takao; Kusuhara, Toshimasa; Mohri, S.; Naruse, Keiji; Kajiya, F.

IFMBE Proceedings. Vol. 17 IFMBE 2007. p. 122-125.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Goda, N, Yamamoto, Y, Kataoka, N, Nakamura, T, Kusuhara, T, Mohri, S, Naruse, K & Kajiya, F 2007, Quantitative evaluation of nano-order micromotion of cultured cells using electric cell-substrate impedance sensing method. in IFMBE Proceedings. vol. 17 IFMBE, pp. 122-125, 13th International Conference on Electrical Bioimpedance and the 8th Conference on Electrical Impedance Tomography 2007, ICEBI 2007, Graz, Austria, 8/29/07.
Goda N, Yamamoto Y, Kataoka N, Nakamura T, Kusuhara T, Mohri S et al. Quantitative evaluation of nano-order micromotion of cultured cells using electric cell-substrate impedance sensing method. In IFMBE Proceedings. Vol. 17 IFMBE. 2007. p. 122-125
Goda, Noriko ; Yamamoto, Y. ; Kataoka, N. ; Nakamura, Takao ; Kusuhara, Toshimasa ; Mohri, S. ; Naruse, Keiji ; Kajiya, F. / Quantitative evaluation of nano-order micromotion of cultured cells using electric cell-substrate impedance sensing method. IFMBE Proceedings. Vol. 17 IFMBE 2007. pp. 122-125
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