Quantitative Estimation of the Effect of Nasal Mucociliary Function on in Vivo Absorption of Norfloxacin after Intranasal Administration to Rats

Daisuke Inoue, Shunsuke Kimura, Akiko Kiriyama, Hidemasa Katsumi, Akira Yamamoto, Ken Ichi Ogawara, Kazutaka Higaki, Akiko Tanaka, Reiko Yutani, Toshiyasu Sakane, Tomoyuki Furubayashi

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Abstract

Nasal drug delivery has attracted significant attention as an alternative route to deliver drugs having poor bioavailability. Large-molecule drugs, such as peptides and central nervous system drugs, would benefit from intranasal delivery. Drug absorption after intranasal application depends on the nasal retention of the drug, which is determined by the nasal mucociliary clearance. Mucociliary clearance (MC) is an important determinant of the rate and extent of nasal drug absorption. The aim of the present study was to clarify the effect of the changes in MC on in vivo drug absorption after nasal application, and to justify the pharmacokinetic model to which the MC parameter was introduced, to enable prediction of bioavailability after intranasal administration. The pharmacokinetics of norfloxacin (NFX) after intranasal administration were evaluated following the modification of nasal MC by pretreatment with the MC inhibitors propranolol and atropine and the MC enhancers terbutaline and acetylcholine chloride. From the relationship between nasal MC and bioavailability after nasal application, prediction of drug absorption was attempted on the basis of our pharmacokinetic model. Propranolol and atropine enhanced the bioavailability of NFX by 90 and 40%, respectively, while the bioavailability decreased by 30% following terbutaline and 40% following acetylcholine chloride. As a result of changes in the MC function, nasal drug absorption was changed depending on the nasal residence time of the drug. On the basis of our pharmacokinetic model, the nasal drug absorption can be precisely predicted, even when the MC is changed. This prediction system allows the quantitative evaluation of changes in drug absorption due to changes in nasal MC and is expected to contribute greatly to the development of nasal formulations.

Original languageEnglish
JournalMolecular Pharmaceutics
DOIs
Publication statusAccepted/In press - Jan 1 2018

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Keywords

  • mucociliary clearance
  • nasal absorption
  • nasal formulation
  • norfloxacin
  • prediction system

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Inoue, D., Kimura, S., Kiriyama, A., Katsumi, H., Yamamoto, A., Ogawara, K. I., Higaki, K., Tanaka, A., Yutani, R., Sakane, T., & Furubayashi, T. (Accepted/In press). Quantitative Estimation of the Effect of Nasal Mucociliary Function on in Vivo Absorption of Norfloxacin after Intranasal Administration to Rats. Molecular Pharmaceutics. https://doi.org/10.1021/acs.molpharmaceut.8b00464