Quantitative assay of epidermal growth factor receptor in human squamous cell carcinomas of the oral region by an avidin-biotin method

T. Kawamoto, K. Takahashi, M. Nishi, Takuji Kimura, T. Matsumura, S. Taniguchi

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A quantitative assay method for epidermal growth factor receptors (EGFRs) of human tumor tissues was established, based on enzyme-labeled avidin-biotin (LAB) interaction with anti-human EGFR monoclonal antibody 528IgG. A standard calibration curve for EGFR estimation in human tumor tissues was obtained with A431#8 cells cloned from A431 human epidermoid carcinoma cell line. The coefficient of variance for the standard curve was below 35% in the application to tumor tissues from nude mice implanted with human tumor cell lines. The minimum tissue amount required for the quantitative assay was around 0.1 g (wet weight). Using the LAB method, the correlation between the level of EGFR number and tumor malignancy was examined for 14 human squamous cell carcinomas (SCCs) from the oral region. Seven of the SCCs showed a more than two-fold higher EGFR number compared to normal gingival tissues. Three highly aggressive carcinomas with poor prognosis possessed five to ten times higher levels of EGFR number than normal tissues. The elevated EGFR level in the SCCs seems to correlate to increasing tumor size and the stage of SCCs as clinically classified according to the 1987 UICC TNM system.

Original languageEnglish
Pages (from-to)403-410
Number of pages8
JournalJapanese Journal of Cancer Research
Volume82
Issue number4
Publication statusPublished - 1991

Fingerprint

Avidin
Biotin
Epidermal Growth Factor Receptor
Squamous Cell Carcinoma
Neoplasms
ErbB Receptors
Tumor Cell Line
Nude Mice
Calibration
Monoclonal Antibodies
Carcinoma
Weights and Measures
Cell Line
Enzymes

Keywords

  • avidin-biotin method
  • epidermal growth factor receptor
  • monoclonal antibody
  • oral cancer
  • squamous cell carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Quantitative assay of epidermal growth factor receptor in human squamous cell carcinomas of the oral region by an avidin-biotin method. / Kawamoto, T.; Takahashi, K.; Nishi, M.; Kimura, Takuji; Matsumura, T.; Taniguchi, S.

In: Japanese Journal of Cancer Research, Vol. 82, No. 4, 1991, p. 403-410.

Research output: Contribution to journalArticle

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AU - Matsumura, T.

AU - Taniguchi, S.

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AB - A quantitative assay method for epidermal growth factor receptors (EGFRs) of human tumor tissues was established, based on enzyme-labeled avidin-biotin (LAB) interaction with anti-human EGFR monoclonal antibody 528IgG. A standard calibration curve for EGFR estimation in human tumor tissues was obtained with A431#8 cells cloned from A431 human epidermoid carcinoma cell line. The coefficient of variance for the standard curve was below 35% in the application to tumor tissues from nude mice implanted with human tumor cell lines. The minimum tissue amount required for the quantitative assay was around 0.1 g (wet weight). Using the LAB method, the correlation between the level of EGFR number and tumor malignancy was examined for 14 human squamous cell carcinomas (SCCs) from the oral region. Seven of the SCCs showed a more than two-fold higher EGFR number compared to normal gingival tissues. Three highly aggressive carcinomas with poor prognosis possessed five to ten times higher levels of EGFR number than normal tissues. The elevated EGFR level in the SCCs seems to correlate to increasing tumor size and the stage of SCCs as clinically classified according to the 1987 UICC TNM system.

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